基于改良方法的光密度值标准化平台开发

Development of Standardization Platform for Optical Density Value Based on an Improved Method

开发一套能够将酶联免疫吸附(ELISA)试验结果标准化的应用平台,以克服ELISA法检测结果变异大,而标准化过程又包含环节多的不足。平台设计以改良光密度值变换法(I-STOD)为基础,标准化公式中的参数估计采用高斯-牛顿迭代法,以程序设计语言VB为平台界面开发工具。结果显示,标准化平台可判断实验结果的有效性,给出可标准化的检测值范围,计算标准化浓度值,对于不在该范围内的样本,给出重新检测时的稀释倍数,规范了标准化工作的流程。该平台界面友好,使用方便,为广大科研人员提供了便捷有效的标准化工具。

Due to the high variation in test results of indirect enzyme-linked immuno sorbent assay （ELISA） and com- plicated steps involved in the process of standardization, a platform used for standardizing the test results from indi- rect ELISA was developed. The platform was designed based on ＇Improved Standardization Method for Optical Den- sity＇ （I-STOD）. Gauss-Newton iteration was applied to estimate parameters in a standard formula. Programming Language VB was used for developing interface of platform. The results indicated that the validity of experiment could be verified through platform. A well determined scope of standardization could be generated. The sample with concentration within the scope was standardized and the degree of dilution was calculated for those outside the scope. The platform was successfully developed which normalized the process of standardization. The function provides the researchers with an effective and convenient tool for quickly achieving standardization of ELISA test results.