子痫前期患者外周血协同刺激分子CD28/CT-LA-4的表达研究

The Expression of CD28 and CTLA-4 in Peripheral Lymphocytes of Preeclampsia Patients

目的:研究子痫前期患者外周血协同刺激分子CD28、CTLA-4、CD28/CTLA-4在CD3+CD4+T细胞和CD3+CD8+T细胞上的表达,探讨CD28/CTLA-4在子痫前期发病中的作用。方法:采集22例正常妊娠妇女(正常妊娠组),45例子痫前期患者(子痫前期组)外周血,用流式细胞技术分别检测外周血T淋巴细胞CD3+、CD4+、CD8+的表达和CD28、CTLA-4、CD28/CTLA-4在CD3+CD4+T细胞和CD3+CD8+T细胞上的表达。结果:子痫前期组外周血T淋巴细胞中CD3+及CD8+的表达低于正常妊娠组(P<0.05),而CD4+及CD4+/CD8+的表达高于正常妊娠组(P<0.05)。与正常妊娠组相比,子痫前期组CD28在CD3+CD4+T细胞和CD3+CD8+T细胞上的表达低于正常妊娠组,但差异无统计学意义(P>0.05);CTLA-4在CD3+CD4+T细胞和CD3+CD8+T细胞上的表达高于正常妊娠组,且重度子痫前期组高于轻度子痫前期组,差异均有统计学意义(P<0.05);CD28/CTLA-4比率在CD3+CD4+T细胞和CD3+CD8+T细胞上的表达低于正常妊娠组,且重度子痫前期组低于轻度子痫前期组,差异均有统计学意义(P<0.05)。结论:子痫前期患者外周血T细胞亚群明显偏移,且协同刺激分子CD28/CTLA-4表达异常,高表达的CTLA-4导致T淋巴细胞亚群失衡,这可能是促使子痫前期发生的重要原因之一。

Objective：To study the alteration of cluster of differentiation 28 （CD28） and cytotoxic T lym- phocyte antigen 4 （CTLA-4） in maternal blood lymphocyte （ CD3 ＋ CD4 ＋ T cell ＋ CD3 ＋ CD8 ＋ T cell） from patients with preeclampsia（PE） and to investigate the function of CD28/CTLA-4 in the pathogenesis of PE. Methods：Peripheral blood from 22 healthy pregnant women （healthy pregnant group）,45 patients with PE （ PE group）. Flow cytometry was used to detect expressions of CD28 and CTLA-4 of peripheral lymphocytes in CD3 ＊CD4 ＋T and CD3 ＋CD8 ＋T cell in the two groups. Results：The expressions of CD3 ＋ and CD8 ＋ in PE group were significantly lower than those in healthy pregnant group（ P 〈0. 05） ,The expressions of CD4 ＋ and CD4 ＋/CD8 ＋ were significantly higher than those in healthy pregnant group （ P 〈 0.05）. There was no difference in the expression of CD28 between preeclampsia group and healthy pregnant group;The expres- sion of CTLA-4 in preeclampsia group was significantly higher than that in healthy pregnant group （ P 〈 0.05） ;The expression ratios of CD28/CTLA-4 in the preeclampsia group were significantly lower than those in healthy pregnant group （P〈0.05）. Conclusions：There is obvious T lymphocyte subsets imbalance and abnormal expression of CD28/CTLA-4 in peripheral lymphocytes of preeclampsia patients. The high exspes- sion of CTLA-4 promotes T lymphocyte subsets imbalance,which might lead to the onset of preeclampsia.