丙酸睾酮致小鼠多囊卵巢综合症动物模型的建立

Testosterone Propionate Induced Polycystic Ovary Syndrome Like Phenotype in Mouse

目的 运用雄激素丙酸睾酮早期注射建立不孕症小鼠动物模型 方法 出生5日龄BALB/c小鼠颈背部皮下注射丙酸睾酮,观察阴道上皮细胞角化情况、交配见栓、妊娠情况、雄激素水平、葡萄糖糖耐量及血清胰岛素测定、卵巢病理切片.结果 模型小鼠出现阴道上皮细胞持续角化、交配见栓率、妊娠率显著低于对照组,血游离睾酮水平较对照组小鼠显著升高（P＜0.05）,模型鼠的基础血糖较对照组有所升高（P＜0.05）,糖代谢正常,模型组胰岛素水平有所升高,但与对照组无显著性差异.卵巢卵泡和正常卵巢相比明显成多囊样发育,见囊状扩张卵泡.结论 丙酸睾酮干预新生小鼠可致雌性小鼠成年后出现交配见栓率低、不孕、血清游离睾酮水平升高,并伴随基础血糖升高,卵巢卵泡呈多囊样改变等多囊卵巢综合症的表现.

Objective To establish the infertility mice model with the phenotype of polycystic ovary syndrome.Method Five-days-old female BALB/c mice were hypodermically injected on the neck and back with androgen testosterone propionate and were fed regularly until to adult.The vaginal epithelial cellular morphology cycle,sexual behavior,pregnancy ability,serum androgen levels,glucose tolerance,serum insulin levels and ovarian pathological changes were observed.Result The challenged female mice showed infertility with the character of refusing sexual behavior and constant vaginal epithelial cell keratinization.Both the testosterone level in serum and the basic blood glucose were higher in the mice than that in the control（P〈0.05）.Furthermore,polycystic ovarian follicle and cystic dilatation follicle were detected in androgen treated mice.Conclusion Hypodermic injection of postnatal female mice with testosterone propionate can sufficiently induces infertility in adult and results in loss of ovulatory cycle and abnormal ovarian pathological changes in animals.The testosterone propionate treated mouse could be used as one of animal model in the research area of polycystic ovary syndrome.