双酚A和4-壬基酚对未成年雌性食蟹猴的生殖内分泌毒性作用

Reproductive Endocrine Toxicity of Bisphenol A and 4-Nonylphenol in Juvenile Female Cynomolgus

目的 研究4-壬基酚（4-NP）和双酚A（BPA）对未成年雌性食蟹猴的拟雌激素作用和内分泌干扰作用.方法 10只16月龄左右的雌性食蟹猴随机分为染毒组和对照组,每组5只.染毒组给予4-壬基酚（4-NP）50 ml/kg＋双酚A（BPA）200 mg/kg联合作用,对照组给予溶剂玉米油,给药剂量为5 ml/kg.每日早晨空腹灌胃,连续4周.每周称重一次,染毒结束时,检测血清雌激素水平、阴道脱落细胞成熟指数,取出子宫,测定其子宫湿重、子宫内膜及环形平滑肌厚度、子宫内膜上皮及腺上皮高度.结果 染毒组与对照组比较血清雌激素水平明显升高,阴道脱落细胞成熟指数显著增加（p＜0.05）,子宫湿重、子宫内膜及环形平滑肌厚度、子宫内膜上皮细胞及腺上皮高度显著增加,差异极显著（P＜0.01）.结论 双酚A和4-壬基酚对未成年雌性食蟹猴具有一定的生殖内分泌毒性作用.

Objective To investigate the estrogen-like activity and endocrinic interference effects of Bisphenol A and 4-Nonylphenol to Juvenile Female Cynomolgus.Methods Ten 16 month old female cynomolgus were randomly assigned to exposure and control groups,each group has 5 animals.The exposure group was given50mg 4-Nonylphenol and 200mg Bisphenol A via oral gavage.Control group was given 5ml of corn oil via oral gavage.Animals were respective dosed before breakfast once daily for 4 weeksThe body weight was recorded once a week.When the study was completed,the serum estrogen level and calculate the maturation index of vagina exfoliative cell was tested.The uterus was collected and the wet weight,endometrial and uterine smooth muscle thickness,endometrial epithelial and glandular epithelium height was measured.Results Compared to control group,the serum estrogen level and maturation index of vagina exfoliative cell were significantly higher in exposure group （P〈0.05）.The uterus wet weight,endometrial and uterine smooth muscle thickness,endometrial epithelial and glandular epithelium height also significantly increased in exposure group （P〈0.01）.Conclusion The Bisphenol A and 4-Nonylphenol have some reproductive endocrine toxic effects to juvenile female cynomolgus.