人热休克蛋白对树突状细胞表面共刺激分子表达影响的研究

Effect of heat shock protein to dendritic cells by the expression of membrane surface molecular

目的:研究人热休克肿瘤抗原复合物对树突状细胞(dendritic cells,DC)表面共刺激分子表达的影响。方法:分离培养人外周血来源的DC细胞,在培养过程中加入分离纯化的人热休克蛋白70(HSP70)抗原复合物及肿瘤细胞裂解物,流式细胞技术分析比较负载HSP70肿瘤抗原复合物、负载肿瘤细胞裂解物及负载前DC细胞表面分子CD80、CD83、CD86及HLA-DR表达水平的变化。结果:培养基中加入HSP70肿瘤抗原复合物后,其表面共刺激分子CD80、CD86和成熟标记分子CD83的表达水平分别为(98.9±1.5)%、(98.3±1.3)%和(53.1±0.8)%,与负载前CD80、CD86和CD83的(66.2±1.6)%、(94.2±2.0)%和(16.3±1.4)%相比显著提高,P=0.013;与肿瘤细胞裂解物负载的DC表面CD80、CD86和CD83的(86.2±1.9)%、(96.5±1.1)%和(55.1±1.0)%相比,共刺激分子CD80的表达显著提高,P=0.020。结论:人热休克肿瘤抗原复合物可提高DC表面共刺激分子的表达水平,促进DC功能活化,有可能在抗肿瘤免疫机制中起重要作用。

OBJECTIVE：To study the effect of human shock protein on dendritic cell（DCs）surface costimulating molecules expression.METHODS：DC cells were isolated and cultured from peripheral blood,loaded by HSP70peptides complex and tumor cells lysates,and then the expression of DC cells membrane surface molecular CD80,CD83,CD86and HLA-DR were detected by flow cytometry before and after loaded.RESULTS：The expression of co-stimulatory molecular CD80,CD86and mature labeled molecular CD83of DC cells loaded with HSP70peptides complex were（98.9±1.5）%,（98.3±1.3）%and（53.1±0.8）%,which were raised by controlled with DC before loaded（66.2±1.6）%,（94.2±2.0）%and（16.3±1.4）%（P=0.013）,and controlled with DC loaded with tumor cells lysates（86.2±1.9）%,（96.5±1.1）%and（55.1±1.0）%.CD80was raised（P=0.020）.CONCLUSION：It is suggested that Hsp70peptide complex can improve the expression of co-stimulatory molecular of DCs,activate DC functions directly,which may possess important effect in immunologic anti-tumor mechanism.