理气开郁法板块方镇痛作用的动态分析

Dynamic Analysis on Analgesic Effect of Regulating Qi and Alleviating Mental Depression Prescriptions

目的:探讨理气开郁法中药板块方的镇痛作用特点。方法:将150只清洁级昆明种小鼠,按体重随机区组法分为15组,包括对照组,柴郁汤、芍甘汤、痛泻要方、金铃子散高中低剂量各组,吗啡和布洛芬组,采用45℃、50℃、55℃水浴引致小鼠疼痛模型,动态测定痛阈潜伏期,用药前测定基础痛阈。采用重复测量设计的方差分析。结果:柴郁汤、芍甘汤和痛泻要方的镇痛作用以高剂量更明显;柴郁汤对45℃、50℃、55℃水浴的痛阈潜伏期均有延长作用,柴郁汤高、中剂量在多个时间点上显示出较好的镇痛作用。结论:柴郁汤的镇痛作用较强,作用特点类似于中枢镇痛药或兼有中枢性镇痛作用;芍药甘草汤、痛泻药方、金铃子散属于作用于外周的镇痛药。

Objective To investigate the characterstics of the analgesic effect of regulating Qi and alleviat ing mental depression prescriptions. Methods One hundred and fifty Kunming mice of clean grade, according to randomized blocks by body weight, were divided into 15 groups, including non-treatment model control group （control group）, high, middle and low dose groups of Chaiyu decotion（柴郁汤 ）, Shaogan decoction（芍甘汤 ）, Tongxie （痛泻 ） prescription and Jinlingzi （金铃子 ） powder, positive control drug Morphine and Ibuprofen groups. A rat pain model was made using tail-immersion in 45 ℃, 50 ℃, 55 ℃ water to induce pain. The pain threshold latency was measured dynamically, measuring the base pain threshold before administration. Analysis of variance of repeated measure definition was applied. Results The analgesic effect of Chaiyu decotion（柴郁汤）, Shaogan decoction （芍甘汤） and Tongxie （痛泻） prescription of high doses was more obvious. Pain thresh old latency was prolonged by Chaiyu decotion（柴郁汤） in tail-immersion test of 45 ℃, 50 ℃, 55 ℃ （P 〈 0.01, P 〈 0.05）, the high and middle doses of Chaiyu decotion（柴郁汤） demonstrated good analgesic effect at multi ple time points （P 〈 0.05）. Conclusion Chaiyu decotion （柴郁汤） has strong analgesic effect, similar to the role of central analgesic drugs; Shaogan decoction （ 芍甘汤）, Tongxie （痛泻） prescription and Jinlingzi （金铃子） powder belong to the peripheral analgesic drugs such as NSAIDs.