阴道炎病原生物检测试剂盒的临床应用价值

Clinical Application Value of Vaginitis Microbe Detection Kits

目的评估阴道炎病原生物检测试剂盒(核酸杂交法)的灵敏度和特异度.方法对400例阴道分泌物标本分别进行Amsel法、滴虫培养、真菌培养和Affirm VPIII检测方法 (核酸杂交法),以Amsel法,滴虫培养,真菌培养为金标准,评价Affirm VPIII检测方法的应用价值.结果与Amsel法相比,Affirm VPIII检测法检测的灵敏度为92.2%,特异度为70.5%;与真菌培养相比,Affirm VPIII检测法检测的灵敏度为88.3%,特异度为92.9%;与滴虫培养相比,Affirm VPIII检测法检测的灵敏度为92.6%,特异度为93.8%;与金标准的一致性较好.结论与传统的检测方法相比,其具备了检测速度快,操作简捷,且一次性可同时区分和识别3种阴道炎致病病原生物,具有较高的敏感性和特异性,具有一定的临床应用价值.

Objective To assess the sensitivity and specificity of vaginitis pathogen detection reagent kit （Nucleic acid hybridization）. Methods Four hundreds cases of vaginal secretion samples were detected with Amsel, vaginalis culture, fungal culture and Affirm VPⅢdetection method, respectively. Using the methods of Amsel, vaginalis culture and fungal culture as the gold standard, the clinical application value of Affirm VPIII detection method was evaluated. Results Compared with Amsel, the sensitivity and specificity of Affirm VPⅢ detection was 92.2% and 70.5%, respectively. Compared with fungal culture, the sensitivity and specificity was 88.3% and 92.9%, respectively. Compared with vaginalis culture, the sensitivity and specificity was 92.6% and 93.8%, respectively. There was a good consistency between the gold stardard and the Affirm VPⅢ detection. Conclusion Compared with the traditional detection methods, the Affir m VPIII detection has the advantages of fast detection speed, simple operation, and high sensitivity and specificity. In addition, it can identify three kinds of vaginitis pathogenic microorganisms at the same time, with a certain clinical value.