不对称咀嚼力作用下CTGF在髁突软骨中的表达

The Expression of CTGF in Condyle Cartilage under Asymmetric Masticatory Force

目的探讨不对称咀嚼肌力对SD大鼠髁突软骨改建的影响.方法通过手术切除一侧颞肌和肉毒神经毒素A注射一侧咬肌,建立不对称咀嚼肌力SD大鼠动物模型,每组6只,分别于建模后3、6、9周处死动物,免疫组织化学检测CTGF在大鼠髁突软骨中的表达.结果 CTGF在髁突软骨增殖层、成软骨细胞层和肥大层表达;实验组CTGF的表达均较空白对照组增强(P<0.05);实验组双侧髁突CTGF表达无明显差异(P>0.05);实验组术后6周CTGF表达强于术后3周,术后9周强于术后6周(P<0.05).结论不对称咀嚼肌力可上调髁突软骨细胞CTGFmRNA的表达,CTGF介导了应力对髁突软骨的改建.

Objective To investigate the effect of the asymmetric of masticatory force on the remodeling of bilateral mandibular condylar cartilage of SD rats. Methods The animal models were established respectively by excising the unilateral temporalis, and injecting botulinum toxin A into the unilateral masseter. Dividing three batch processing 3 weeks, 6 weeks, and 9 weeks after the animal modeling, six rats were killed in each group. The expression of CTGF in condylar cartilage was observed by immunohistochemical staining. Results CTGF expressed in proliferative layer,chondroblast layer and hypertrophic layer of the condylar cartilage. The expression of CTGF in the experimental groups was increased than control groups （〈0.05） . There was no significant difference between the left and right side in the experimental groups （ 〉0.05）, the expression of CTGF after 6 weeks of operation was stronger than the group after 3 weeks of operation, the expression of CTGF after 9 weeks of operation was the strongest than all of other groups （ 〈0.05） . Conclusions The asymmetric of masticatory force can up-regulate the expression of CTGF mRNA in rat mandibular condylar chondrocytes. CTGF induces the effect of stress-mediated on the mandibular condylar cartilage remodeling.