白花蛇舌草提取物对结肠癌细胞抑制作用的实验研究

Inhibition of Colon Cancer Cells by Ethanol Extract of Oldenlandia Diffusa

目的探讨白花蛇舌草乙醇提取物对BALB/c小鼠CT-26结肠癌细胞株增殖的抑制作用.方法采用甲基噻唑蓝(MTT)法测定不同浓度白花蛇舌草乙醇提取物对CT-26细胞株的生长抑制作用,计算半数抑制浓度(IC50).结果在同一浓度下,白花蛇舌草乙醇提取物对CT-26细胞的抑制作用随着作用时间的延长而增强,0.08 mg/mL的白花蛇舌草乙醇提取物作用CT-26细胞24 h、48 h、72 h,细胞抑制率分别为(16.67±9.35)%,(34.66±9.23)%,(40.07±9.16)%.白花蛇舌草乙醇提取物作用24 h、48 h、72 h的IC50值分别为0.315,0.155,0.115.在同一作用时间内,白花蛇舌草乙醇提取物对CT-26细胞的抑制率随着药物浓度的增加而增加,在72 h内不同浓度药物(0.06 mg/mL,0.08 mg/mL,0.10 mg/mL,0.12 mg/mL,0.14 mg/mL,0.16 mg/mL,0.18 mg/mL,0.20 mg/mL)作用下,细胞抑制率分别为(35.46±3.59)%,(40.07±9.16)%,(40.77±6.92)%,(52.81±1.87)%,(54.22±2.35)%,(68.72±3.71)%,(70.04±8.03)%,(71.84±3.12)%.结论白花蛇舌草乙醇提取物可以抑制BALB/c小鼠CT-26结肠癌细胞株的增殖,其对CT-26细胞增殖的抑制作用呈现时效和量效的关系.

Objective To explore the inhibitory effect of the ethanol extract of Oldenlandia diffusa on the proliferation of CT-26 colon cancer cells which come from BALB/c mice. Method We determined the inhibitory effect of different concentrations of ethanol extract of Oldenlandia diffusa on CT-26 cells＇ proliferation by using methyl thiazolyl tetrazolium （MTT）, and calculated the 50% inhibiting concentration （IC50） . Results As to the same concentration, the inhibitory effect of the ethanol extract of Oldenlandia diffusa on CT-26 cells was increased with time, for exsample：after treated with 0.08 mg/mL of ethanol extract of Oldenlandia diffusa for 24 h, 48 h and 72 h, the inhibitory rates of CT-26 cells were （16.67 ±9.35）%, （34.66 ±9.23）%and （40.07 ±9.16）%, respectively. After treating CT-26 cancer cells for 24 h, 48 h and 72 h, the IC50 values of the ethanol extract of Oldenlandia diffusa were 0.315,0.155 and 0.115 mg/mL, respectively. In the same treatment time, the inhibitory effect of the ethanol extract of Oldenlandia diffusa on CT-26 cells was increased with the increase of concentration：after treatment for 72 h with different concentrations （0.06 mg/mL,0.08 mg/mL,0.10 mg/mL,0.12 mg/mL, 0.14 mg/mL,0.16 mg/mL,0.18 mg/mL and 0.20 mg/mL） of the ethanol extract of Oldenlandia diffusa,the inhibitory rates of CT-26 cells were （35.46 ±3.59）%, （40.07 ±9.16）%, （40.77 ±6.92）%, （52.81 ±1.87）%, （54.22±2.35）%, （68.72±3.71）%, （70.04±8.03）%and （71.84±3.12）%, respectively. Conclusion The ethanol extract of olenlandia diffusa can inhibit the proliferation of CT-26 colon cancer cells from BALB/c mice in a time and dose dependent manner.