半乳糖凝集素-1在小鼠病毒性心肌炎中的动态变化

The dynamic change of galectin-1 in in mice with viral myocarditis

目的探讨半乳糖凝集素-1(galectin-1)在病毒性心肌炎(VMC)小鼠心肌细胞中表达的动态变化。方法选取雄性BALB/c小鼠58只,随机分为病毒组50只、对照组8只。病毒组小鼠腹腔接种TCID50为10-5/L的Nancy株CVB3病毒液0.10 ml,并于接种后第7、10、14和28天,分别随机处死8只小鼠;对照组小鼠腹腔接种不含病毒eagle液0.10 ml,于接种后第28天处死。光镜下观察小鼠心肌组织的病理变化,ELISA法检测外周血中galectin-1水平,实时荧光定量聚合酶链反应(RQ-PCR)检测心肌组织中galectin-1 mRNA的表达。结果与对照组比较,病毒组小鼠心肌组织galectin-1 mRNA的表达在第7、10天均增高,差异均有统计学意义(P均<0.05);病毒组小鼠外周血galectin-1水平在第7天、10、14天亦增高,差异均有统计学意义(P均<0.05)。结论 VMC小鼠心肌细胞中galectin-1的表达呈动态变化,galectin-1在VMC的发生发展中可能起一定作用。

Objectives To investigate the dynamic change of galectin-1 in myocardium of mice with viral myocarditis （VMC）. Methods A total of 58 male BALB/c mice were selected and randomly divided into CVB3 group （n=50） and control group （n=8）. Mice in CVB3 group were infected with 0.10 ml 105/L CVB3 through intraperitoneal inoculation, and 8 mice were killed on day 7, 10, 14 and 28 respectively after inoculation. Mice in control group inoculated with 0.10 ml eagle reagent was killed on day 28. The myocardial pathological changes were observed using light microscope. In addition, expressions of serum galectin-1 were detected by ELISA and expressions of myocardial galectin-1 were detected by real-time quantitative-polymerase chain reaction （RQ-PCR）. Results Compared with control group, the myocardial expression of galectin-1 mRNA in CVB3 group was obviously increased on day 7 and day 10 （all P〈0.05）; the serum concentration of galectin-1 in CVB3 group was obviously increased on day 7, day 10 and day 14 （all P〈0.05）. Conclusion The expressions of galectin-1 in myocardium of mice with viral myocarditis were in dynamic changes. Galectin-1 may play a role in the pathogenesis and development of viral myocarditis.