缺氧诱导因子-1α蛋白在外阴癌组织中的表达及临床意义

Expressions and clinical significance of HIF-1α in vulvar cancer tissues

目的:检测缺氧诱导因子-1α(HIF-1α)蛋白在外阴癌组织和外阴上皮内瘤病变组织中的表达,探讨其与临床病理特征和疾病预后的关系。方法:选择手术切除外阴癌组织标本48例,外阴上皮内瘤病变组织14例,正常外阴组织19例。采用免疫组织化学SP法检测上述组织中HIF-1α蛋白的表达情况。结果:HIF-1α在外阴癌中阳性率(58.3%)显著高于正常外阴组织(5.3%)、外阴上皮内瘤变(14.3%),P<0.05。单因素分析表明有淋巴转移组的HIF-1α阳性率(78.6%)显著高于无淋巴转移组(35.3%),P<0.05。Ⅰ-Ⅱ期组的HIF-1α阳性率(47.6%)显著低于Ⅲ-Ⅳ期组(77.8%),P<0.05。中、低分化外阴癌中HIF-1α阳性率(63.0%)高于高分化癌(28.6%),P<0.05。结论:HIF-1α表达与外阴癌的淋巴转移、临床分期等显著相关。

Objective： To detect the expression levels of hypoxia - inducible factor - 1α protein （ HIF - 1α ） in vulvar carcinoma（VC） paraffin embedded tissues, and explore their relationship with the clinieopathological features and prognosis. Methods：Immunuohistochemical SP method was applied to detected the expression of HIF - et in 48 cases with VC, 14 cases with vulvar intraepithelial neoplasia（ VIN ） and 19 cases of normal vulvar tissues. Results： The positive rate of HIF -lct in VC（58.3% ） was significantly higher in VC than those in normal tissue（5.3% ） and VIN （ 14.3% ） （ P 〈 O. 05 ）. Univariate analyse showed that the positive rates of HIF - lot in VC r tissues with lymph node metastasis （78.6%） were significantly higher than that in VC without metastasis（35.3% ） （ P 〈0.05）. HIF -lct - positive rate in VC tissues with stage of I - II（47.6% ） was significantly lower than that in stage of m - Iv group （77.8%） （ P 〈0.05）. Also the HIF - lot - positive rate in poorly differentiated vulvar cancer （63.0%） was signif- icant higher than that in well - differentiated carcinoma（ 28.6% ） （ P 〈 0.05 ）. Conclusion：The HIF - 1α expression is correlated with lymphatic metastasis and clinical stage in VC tissue.