全血MicroRNA-126—急性心肌梗死的新标记物

Whole blood MicroRNA-126—a new biomarker of acute myocardial infarction

目的:近年MicroRNAs作为诊断急性心肌梗死的一类新生物标记物成为研究的热点。本研究对MicroRNA-126(miR-126)在急性心肌梗死急性期的表达进行了检测。方法:本研究纳入急性心肌梗死(acute myocardial infarction,AMI)患者36例,正常对照组患者34例。在心肌梗死症状发作后的不同时间段:AMI患者入院时(T0),AMI发病8 h(T8),AMI发病12 h(T12),AMI发病24 h(T24),AMI后第7天采集血液样本并应用实时定量PCR法对全血中miR-126表达水平进行检测。应用酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)法检测血浆肌钙蛋白I(plasma troponin I,cTnI)的表达水平。结果:急性心肌梗死患者全血中miR-126的表达水平在T0、T8、T12、T24时间点均较对照组明显升高且有统计学意义(P<0.05);而AMI后第7天时其表达水平与对照组无明显差异且无统计学意义(P>0.05)。AMI早期全血miR-126与cTnI的显示了相同的升高趋势。受试者工作特征曲线(ROC曲线)分析结果显示全血miR-126在T0、T8、T12、T24时间点的ROC曲线下面积(area under curve,AUC)分别为0.857,0.863,0.827,0.789(P值均<0.05)。结论:在心肌梗死早期miR-126在AMI早期表达水平明显升高,且与cTnI相同的时间窗,但拥有更早的达峰时间。这提示检测全血miR-126可能作为早期诊断急性心肌梗死的有效方法。

Objective：In recent years,as a new biological marker in the diagnosis of acute myocardial infarction MicroRNAs become a hot research. The study investigates the expression of MicroRNA-126 （miR-126） in the acute phase of acute myocardial infarction. Methods： The study included 36 patients in the group of acute myocardial infarction （AMI） ,while 34 patients in the normal control group. In the different periods： the time of AMI patients with admission to hospital （TO） ,AMI incidence 8 h （T8） , AMI incidence 12 h （T12） ,AMI incidence 24 h （T24） ,AMI 7 days after the collection of blood samples and apply real-time quantitative PCR method to detect the expression level of miR-126 in whole blood. Enzyme-linked immunosorbent assay （ELISA） is used for detecting plasma tro- ponin I （cTnI） expression levels. Results： The expression levels of whole blood miR-126 of patients with acute myocardial infarction at T0,T8, TI 2,T24 time points are significantly higher than those of the control group and was statistically significant （P 〈 0.05 ） ;the expression level after 7days shows no difference with the control group and there is no statistical significance （P 〉 0.05）. In the early stages of AMI, MiR-126 and eTnI in whole blood show the same increasing trend. The receiver operating characteristic curve （ROC） shows that the area under curve （AUC） of whole blood miR-126 at the T0,T8 ,T12,T24 time points were 0. 857,0. 863,0. 827 ,and 0. 789 （P values 〈 0.05 ）. Conclusion： In the early stage of myocardial infarction AMI,the expression level miR-126 was significantly higher,with the same time window but earlier peak time with cTnI. It is suggested that the whole blood miR-126 may be used as an el-fective method for early diagnosis of acute myocardial infarction.