EGFR、Kras、BRAF在喉鳞癌中的表达及临床意义

Expressions and clinical significance of EGFR,Kras and BRAF in laryngeal squamous cell carcinoma

目的:探测EGFR及Ras-Raf信号通路在喉鳞状细胞癌组织中的表达,探讨其在喉癌发病机制中的意义。方法:采用免疫组化SP法检测58例喉鳞癌组织及20例癌旁正常的喉黏膜组织中的EGFR、Kras、BRAF的表达,分析它们与喉鳞癌临床病理因素之间的相关关系。结果:EGFR、Kras、BRAF在喉癌组织中的阳性表达率,分别为74.14%(43/58),25.86%(15/58)和39.66%(23/58),在癌旁黏膜中的阳性表达率,分别为40%(8/20),15%(3/20),15%(3/20),EGFR、BRAF在喉癌组织中的表达高于在癌旁黏膜中的表达,差异有统计学意义(P<0.05),Kras在喉癌组织与癌旁黏膜中的表达差异无统计学意义(P>0.05)。EGFR、BRAF分别与组织学分化、淋巴结转移和/或预后相关(P<0.05);Kras与临床病理因素无关(P>0.05);三者之间无相关性(P>0.05)。结论:喉鳞癌组织EGFR、BRAF的过表达可能与喉癌的发生发展有关,Kras与喉癌的关系尚不明确;Ras-Raf信号通路中,抗EGFR、BRAF靶向治疗对喉癌患者有益,而抗Kras对喉癌的靶向治疗效应有待进一步研究。

Objective：To detect the expression of EGFR and the Ras-Raf signal pathway in laryngeal squamous cell carcinoma,and explore its significance in laryngeal cancer pathogenesis. Methods：Detect the expression of EGFR ~ Kras and BRAF from 58 cases of la- ryngeal squamous cell carcinoma and 20 cases of adjacent normal laryngeal mucosa by immunohistochemical SP method, and analyze the correlation between the clinic pathology factors and laryngeal squamous cell carcinoma. Results： The positive rates of EGFR, Kras and BRAF in laryngeal squamous cell carcinoma were 74.14% （43/58） ,25.86% （ 15/58 ） and 39. 660/＊ （23/58） , while the positive rates were 40% （8/20） , 15% （3/20） , 15% （3/20） in adjacent noncancerous mucosa, The expressions of EGFR and BRAF in laryngeal car- cinoma were higher than that in adjacent noncancerous mucosa. The difference shows statistical significance（ P 〈 0.05 ） ; the expression of Kras in laryngeal carcinoma and adjacent noncancerous mucosa showed no statistical significance（ P 〉 0.05 ）. The expression of EC,- FR and BRAF are correlated with lymph node metastasis ~pathological grade and/or prognosis （P 〈 0.05 ）. The expression of Kras had no correlation with all the clinical factors mentioned above. There is no correlation among EGFR, Kras and BRAF expression （ P 〉 0. 05 ）. Conclusion ： The over expressions of EGFR and BRAF have a certain correlation with the genesis and development of the la- ryngeal squamous cell carcinoma,and the correlation between Kras and laryngeal cancer is not clear;Ras-Raf signal pathway may pro- vide benefits for the anti-EGFR and anti-BRAF targeted therapy of laryngeal cancer. But the anti-Kras targeted therapy of laryngeal cancer needs to be further studied.