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酸性或碱性食品沙门氏菌检测前增菌简化实验研究

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摘要 GB4789.4-2010《食品安全国家标准食品微生物学检验沙门氏菌检验》方法中规定,待检样品需测定pH值,用1mol/mL无菌NaOH或HCl调pH值军6.8±0.2,但从前增菌液缓冲蛋白冻水(BPW)成分分析,发脱其中缓冲剂磷酸氯二钠和磷酸二氢钾正好构成了磷酸盐缓冲体系,可以自身通过调整电离方向达到电离平衡,从而维持了增菌液的pH值,这样低酸或低碱样品住加入BPW前就不用调pH值,实验论证了该理论成立的可能性,并说明了检测样品的pH值的范围及简化优点,致使低酸或低碱性性食品检测不需要调整pH值直接增菌检测沙门氏菌,简化了操作步骤,还避免调pH值引起的外界污染,达到操作简便、快捷的目的。
出处 《中国食品工业》 2013年第9期50-51,共2页 China Food Industry
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