两种锦鸡和环颈雉线粒体DNA(mtDNA)的比较研究

POLYMORPHISM IN THE mtDNA OF THREE SPECIES OF PHEASANTS

本文以10种限制性内切酶分析雉科中环颈雉(Phasianus colchicus),红腹锦鸡(Chrysolopyus pictus)和白腹锦鸡(Chrysolophus amherstiae)线粒体DNA(mtDNA)。雉属与锦鸡属之间的遗传距离P为0.076(0.061—0.085),红腹锦鸡与白腹锦鸡的P为0.012。推算雉属和锦鸡属的分化大约发生在3.8×10~6年以前,红腹锦鸡与白腹锦鸡的分化大约在6×10~5年以前。这些结果表明:1.在雉科系统发生中,雉属与锦鸡属是近缘的属;2.红腹锦鸡和白腹锦鸡的分化较晚,关系密切,可能只是两个亚种。

Mitochondrial DNA (mtDNA) polymorphisms in three species of pheasants, i. e., common pheasant (Phasianus colchicus), golden pheasant (Chrysolopus pictus) , and Chinese pheasant (C. amherstiae) , were analyzed to study phylogenetic relationships, when 10 restriction enzymes of 6-bp recognition were used, 20-29 sites were observed in the samples. The estimated number of nucleotide substitutions per site ( p ) between genera Phasianus and Chrysolopus is 0.076 (rangs from 0.067-0.085), and between golden and Chinese pheasants is 0.012. Divergence times between the two genera, and between the two species of genus Chrysolopus were estimated to be 3.8x106 and 6 X 105 years on the mean rate of sequences divergence of 0.02 per million years in mtDNA, respectively. These findings suggest: 1. Phasianus and Chrysolopus are two closely related genera in the phylogeny of pheasants. 2. There is possible that golden and Chinese pheasants are two subspecies, not two species.