摘要
目的:筛选制备8-甲氧基补骨脂素(MOP)柔性纳米脂质体的合适方法。方法:根据正交实验设计及预试验,确定基本配方大豆磷脂∶胆固醇∶MOP∶胆酸钠=20∶10∶3∶10。分别采用逆相蒸发法、乙醚注入法、薄膜法、薄膜-超声法等方法制备MOP柔性纳米脂质体,比较脂质体包封率、平均粒径和Zeta电位。结果:逆相蒸发法制备的脂质体包封率低,粒径大;乙醚注入法包封率低且平均粒径小;薄膜法包封率高,粒径大;薄膜-超声法包封率高,平均粒径适中,且Zeta电位负值较高;超声15min获得的脂质体包封率较高,平均粒径大小合适,Zeta电位的负值也比较高。结论:采用薄膜-超声法,超声时间15min是制备补骨脂素柔性纳米脂质体比较适宜的方法。
Objective：Screening suitable method for preparing flexible nano-liposome of 8- methoxypsor- Men. Methods：According to orthogonal experimental design and pre test the basic recipe was determined： soybean lecithin：cholesterol：MOP： sodium cholate= 20：10：3：10. MOP flexible nano-liposomes were prepared by using ei ther reverse-phase evaporation method, aether infusion method, film-dispersion or film-dispersion- ultrasound meth od. Liposomal envelopment efficiency, the average particle size and zeta potential were compared between different methods. Results：Reverse-phase evaporation method has low envelopment efficiency and large particle size; aether infusion method has low envelopment efficiency and small particle size; film-dispersion method has high envelopment efficiency and large particle size; film-dispersion ultrasound method has high envelopment efficiency and suitable par tiele size and zeta potential. Conclusion：The film ultrasonic method with ultrasonic time of 15 minutes for preparing MOP flexible nano liposome is a more appropriate method.
出处
《陕西医学杂志》
CAS
2013年第10期1290-1292,共3页
Shaanxi Medical Journal
基金
广东省科技计划项目(2010B030700054)
