TCT联合高危HPV-DNA定量检测在宫颈疾病筛查中的价值

The Value of TCT combined with high-risk HPV-DNA quantitative detection in screening of cervical diseases

目的通过液基细胞学(TCT)联合高危型(8个型)人乳头瘤病毒DNA(HR-HPV-DNA)定量检测,探讨其在宫颈疾病筛查中的价值。方法对怀疑有宫颈病变的患者进行(TCT)和HR-HPV-DNA(16,18,31,33,45,52,56,58型)实时荧光定量检测,并将两者结果和病理活检进行对照分析。结果 TCT检测与HR-HPV-DNA检测特异性比较有显著差别(P<0.01),HR-HPV-DNA检测诊断敏感度高(92%),但特异性低(75%);TCT检查特异性高(97%),敏感性低(83%);TCT与联合筛查检出率高达100%,两组比较有明显差别(P<0.01)。结论 TCT联合HR-HPV-DNA进行宫颈疾病的筛查,能明显提高检出率和筛查效率,在宫颈癌高危人群中进行大规模的初筛更具价值,适合在条件允许的地方进行开展。

Objective To explore the value of TCT in combination with quantitative detection of high - risk type human papillomavirus （HPV） DNA （8 types） in screening cervical diseases. Methods Suspected patients with cervi- cal lesion were examined with TCT and the high risk HPV -DNA （type 16,18,31,33,45,52,56, and 58） by using real -time fluorescence quantitative detection, and both results and pathological biopsy were analyzed comparative- ly. Results There was significant difference （ P 〈 0.01 ） between TCT detection and high risk HPV - DNA detection in the specificity. The sensitivity of high risk HPV - DNA diagnosis is higher （ 92% ）, but specificity is lower （75%） ; whereas the specificity of TCT is higher （97%）, but sensitivity is lower （83%） ; TCT combined with high risk HPV -DNA detection could increase the rate of detecting cervical diseases to 100% ,and the comparison of two groups was significantly different （P 〈 0.01 ）. Conclusion TCT combined with quantitative detection of high risk HPV- DNA for screening cervical diseases can significantly improve detection rate and screening efficiency, which is more valuable to carry out on a large scale screening for cervical cancer in high - risk populations at early stage and suitable in places where conditions permit.