斑蝥酸钠维生素B_6对人肝星状细胞增殖的影响及其机制

Influence and mechanism of disodium cantharidinate and vitamin B_6 injection on the proliferation of human hepatic stellate cells

目的探讨斑蝥酸钠维生素B6注射液对人肝星状细胞LX-2增殖活化的影响及其机制。方法培养人肝星状细胞LX-2,将其分为3个观察组(斑蝥酸钠维生素B6的浓度分别为1、5、10μg/mL)和1个空白对照组;培养24、48、72 h后,采用MTT法检测细胞增殖活性,采用荧光定量PCR方法检测Ⅰ型胶原、Ⅲ型胶原、转化生长因子β1基因表达变化。结果与空白对照组比较,观察组人肝星状细胞LX-2增殖活化被显著抑制(P均<0.05),Ⅰ型胶原、Ⅲ型胶原、转化生长因子β1mRNA表达下降(P均<0.05)。结论斑蝥酸钠维生素B6可以抑制人肝星状细胞LX-2增殖活化,其机制可能与其抑制、转化生长因子β1信号通路有关。

Objective To study the influence and mechanism of disodium cantharidinate and vitamin B6 injection on the proliferation and activation of human hepatic stellate cells LX-2. Methods The human hepatic stellate cells LX-2 were cultured and divided into 4 groups ： the control group, three experimental groups treated with different concentrations of disodium cantharidinate （ 1, 5 and 10 p.g/mL, respectively）. The proliferative activities of LX-2 were detected by MTF after 24 h, 48 h and 72 h. The expression changes of COL Ⅰ , COL Ⅲ and TGF-131 mRNA in LX-2 cells were determined by FQ-PCR; and the expression changes of COL Ⅰ , COL Ⅲ and TGF-β1 protein in LX-2 cells were determined by Western blot. Results Compared with the control group, the proliferation and activation of human hepatic stellate cells LX-2 in the three experimental groups were significantly inhibited （ all P 〈 0.05 ） , and the expression of COL Ⅰ , COL Ⅲ and TGF-β1 mRNA in LX-2 cells were decreased （ all P 〈 0.05 ）. Conclusion Disodium cantharidinate and vitamin B6 injection can inhibit the proliferation and activation of LX-2 cell line, whose mechanism might be correlated with the inhibition of TGF-β1 signal pathway.