^(89)Sr照射对乳腺癌MCF-7细胞分泌sFas、sFasL的影响

Effects of ^(89)strontium irradiation on sFas and sFasL excreted from breast cancer MCF-7 cells

目的探讨89Sr照射对乳腺癌MCF-7细胞分泌sFas、sFasL的影响。方法采用不同浓度89Sr照射乳腺癌MCF-7细胞,培养24 h后,用酶联免疫吸附试验检测细胞培养上清液中的sFas、sFasL水平。结果乳腺癌MCF-7细胞分泌sFas、sFasL;89Sr照射可明显抑制MCF-7细胞增殖,随着89Sr浓度升高,其sFas、sFasL水平逐渐下降,89Sr不同浓度间的sFas、sFasL水平两两比较,P均<0.05。结论89Sr可诱导乳腺癌MCF-F细胞凋亡,89Sr照射能有效降低MCF-7细胞分泌sFas、sFasL的水平;sFas的动态变化可能与乳腺癌细胞发生、发展的病理生理过程有关。

Objective To investigate the effects of 89strontium （89Sr） irradiation on the levels of sFas and sFasL excreted from breast cancer MCF-7 cells. Methods MCF-7 cells were irradiated by different concentrations of 89Sr and cultured for 2d h. Enzyme-linked immunosorbent assay（ELISA） was applied to detect the levels of sFas and sFasL in the cell culture supernate. Results The breast cancer MCF-7 cells excreted sFas and sFasL. 89Sr irradiation had significant inhibitory effect on the proliferation of MCF-7 cells. The levels of sFas and sFasL decreased when the concentrations of 89sr increased. The levels of sFas and sFasl were significantly different between different concentrations of 89Sr irradiation （ all P 〈 0. 05）. Conclusions Apoptosis of breast cancer MCF-7cells can be induced by 89Sr. The levels of sFas and sFasL excreted from MCF-7 decreased when irradiated by 89Sr. The dynamic changes in levels of sFas and sFasL may be associat- ed with the occurrence, development of breast cancer pathogenesis.