原因不明不孕症患者种植窗期子宫内膜容受性变化及意义

Alteration of endometrial receptivity in the implantation window of unexplained infertility

目的探讨原因不明不孕症患者种植窗期子宫内膜容受性（ER）变化及意义。方法选择原因不明不孕症患者30例（观察组）及因男方因素致不孕症的正常女性30例（对照组），均于月经周期第19～24天行子宫内膜活检，采用sP免疫组化法检测两组子宫内膜中的IL-11、白血病抑制因子（LIF）、信号转导与转录活化因子3磷酸化（pSTAT3）表达，并分析各指标问的相关性。结果观察组子宫内膜中的LIF、IL-11、pSTAT3表达均低于对照组（P均〈0．05）；相关分析显示，LIF与IL-11、IL-11与pSTAT3（腺上皮）、pSTAT3（腺上皮）与LIF之间均有显著正相关性（r分别为0．489、0．803、0．573，P均〈0．01）。结论原因不明不孕症患者种植窗期子宫内膜中的LIF、IL-11及pSTAT3表达存在缺陷，可导致胚胎种植能力下降。

Objective To investigate the alteration of endometrial receptivity （ER） in the implantation window of unexplained infertility and its significance. Methods Endometrial biopsies （in the menstrual cycle, 19 to 24 days ） were conducted in 30 women with unexplained infertility （observation group） and 30 healthy women with male factor infertility （control group）. The expression of IL-11, phosphorylated signal transducer and activator of transcription 3 （pSTAT3）, and leukemia inhibitory factor （LIF） was determined by immunohistochemical SP method, meanwhile the correlation analysis was conducted between each index. Results The expression of LIF, IL-11 and pSTAT3 in the observation group was significantly lower than that in the control group （ all P 〈 0.05 ）. And there were positive correlations between LIF and IL-ll,IL-11 and pSTAT3, LIF and pSTAT3 （r=0.489,0.803 and0.573, respectively; allP〈0.01）. Conclusion There are defects of LIF, IL-11 and pSTAT3 expression in the endometrium of implantation window of unexplained infertility, which may contribute to the decline of embryo implantation ability.