防风通圣散干预非酒精性脂肪肝大鼠实验研究

Experimental Study of Fang-feng Tong-sheng Powder on Non-alcoholic Fatty Liver in Rats

目的:研究防风通圣散干预非酒精性脂肪肝实验大鼠模型的影响。方法:取成年SD雄性大鼠60只,适应性饲养2周后,随机分为2组:正常对照组12只(予普通饲料),造模组48只(予高脂饲料)。在给予高脂饮食后的第2周开始,将造模组的48只SD大鼠随机分为模型对照组12只,防风通圣散低、中、高剂量治疗组各12只。至造模10周末,防风通圣散低、中、高剂量治疗组分别给予防风通圣散生药6,12,16 g·kg-1ig qd干预2周。各组饲养方式不变。然后静脉取血进行生化检查,处死动物,取新鲜肝脏制备标本。结果:与正常对照组比较,模型对照组大鼠血清肝功能、血脂指标,肝细胞脂肪变程度均有明显的升高、加重(P<0.01)。与模型对照组比较,防风通圣散低、中、高剂量组大鼠血清肝功能和血脂指标,肝细胞脂肪变程度均有统计学差异(P<0.01)。结论:防风通圣散干预非酒精性脂肪肝能明显恢复肝组织的结构,减轻肝细胞的损伤,保护肝功能,降低血脂参数。

Objective： To study the effect of Fang -feng Tong -sheng Powder on nonalcoholic fatty liver in experimental rat model. Methods： 60 male SD adult rats were randomly divided into two groups after 2 weeks＇ adaptive feeding, in which 12 ones were in normal control group （given to ordinary feed） , and 48 ones in model - making group （ given to high fat feed）. At the second week start of a high - fat diet, 48 SD rats were randomly divided into model control group （ 12 rats） and treatment groups of low, medium and high dose Fang -feng Tong -sheng Powder. Until the 10th week Fang-feng Tong-sheng Powder in low, medium and high dose were respectively given to treatment groups： 6, 12, 16 g · kg^-1 ig qd, and the administration had been for 2 weeks. Then the venous blood was drew to do biochemical examination, the rats were executed, and fresh liver were prepared into animal specimens. Results： Compared with in normal control group, liver function, blood lipid and liver steatosis degree were increased markedly and aggravatingly in model control group （ P 〈 0.01 ）. Compared with the model group, the difference was statistically significant in treatment groups on liver function, blood lipid and liver steatosis degree （P 〈 0.01 ）. Conclusion： Fang-feng Tong-sheng Powder can obviously restore liver tissue, reduce the damage of liver cell, protect the liver function, and reduce blood lipid parameters on non - alcoholic fatty liver disease.