盐生植物盐穗木悬浮细胞系的建立

Establishment of Suspension Cell Line of Halostachys caspica

【目的】组织和细胞培养能够为研究盐穗木的耐盐机制提供实验材料,建立盐穗木悬浮细胞系,从细胞水平上揭示盐生植物盐穗木的耐盐机制。【方法】以成熟胚为外植体,探讨不同培养基和激素配比对愈伤组织诱导以及悬浮细胞系培养条件的优化,并以酚藏花红染色和固体培养基培养法检测悬浮细胞的细胞活性。【结果】以葡萄糖替换蔗糖作为培养基碳源,在0.5 mg/L 6-BA、1.0 mg/L KT下盐穗木诱导愈伤组织最适宜悬浮细胞的培养;而在液体基础培养基中添加1.0 mg/L 6-BA、1.0 mg/L KT,则最有利于盐穗木悬浮细胞系的稳定。所获得的盐穗木悬浮细胞具有一定的细胞活性。【结论】采用成熟胚为外植体诱导愈伤组织能够获得盐穗木悬浮细胞系,为在细胞层面上深入开展盐穗木耐盐机制奠定基础。

[ Objective ] The purpose of this program was to establish suspension cell line of Halostachys caspica,which contributes to the elucidation of salt - tolerance mechanism of H. caspica at the cellular level with molecular genetic techniques. [ Method ] The mature embryos of H. caspica as explants were used to ex- plore the impact of different medium or different concentration of hormone combinations on callus induction and suspension cell culture. And the cells were respectively stained with phenosafranine and sub -clutured on the solid medium to detect the cell viable ability of H. caspica suspension ceils. [ Result ] As a result, the optimal medium for callus induction was the one containing 0.5 mg / L 6 - BA and 1.0 mg / L KT, together with glu- cose substituted for sucrose as carbon source, while the ratio of hormone （ 1.0 mg / L 6 - BA and 1.0 mg / L KT） is the fittest for suspension cell culture of H. caspica. And the suspension ceils had certain cell viability. [ Conclusion ] Suspension cell line of the H. caspica was successfully gotten with callus induced from the ma- ture embryos of H. caspica ,which laid the foundation for the research into the salt tolerance mechanism of H. caspica at the cellular level.