2型糖尿病及大血管病变患者CD4^+CD28^-T细胞、CD4^+CD25^+T细胞亚群的变化及意义

Changes and significance of CD4^+CD28^-T cells,CD4^+CD25^+T cells in type 2diabetes and its macrovascular complications

目的:观察外周血CD4+CD28-T细胞、CD4+CD25+T细胞亚群在2型糖尿病患者及大血管病变患者的变化,探讨其在2型糖尿病及大血管病变发病中的作用。方法:应用免疫荧光标记技术和流式细胞仪,检测36例初诊2型糖尿病(单纯DM组)患者和28例糖尿病并大血管病变患者、20例健康对照者的外周血T细胞亚群变化。结果:DM并大血管病变组、单纯DM组CD4+CD28-T细胞明显高于健康对照组,差异均有统计学意义(P<0.05);而CD4+CD25+T细胞明显低于健康对照组(P<0.05);DM并大血管病变组CD4+CD28-T细胞高于单纯DM组(P<0.05),CD4+CD25+T细胞低于单纯DM组。结论:机体细胞免疫异常在2型糖尿病及大血管病变的发生、发展中发挥着一定的作用。

Objective：To investigate the changes of CD4＋CD28-T cells, CD4＋CD25＋T cells and their roles in the development of type 2 diabetes and its macrovascular complications. Methods： T lymphocyte subsets of periphera1 blood mononuclear cells （PBMCS） were determined with immunofluorescence staining and flow cytometry method from 36 patients with type 2 diabetes mellitus and 28 patients with macrovascular disease, 20 healthy controls. Results：The percentage of CD4＋CD28-T cells increased while CD4＋CD25＋T cells were decreased in those with type 2 diabetes and macrovascular disease （P〈0.05）.Al1 of the above changes had significantly difference from those in control group （P〈0.05）. CD4＋CD28-T cells in the group of macrovascular disease were also higher than those in type 2 diabetes and CD4＋CD25＋T cells were lower. Conclusion：Immune dysfunction exerts have certain effects on the pathogenesis and progression of type 2 diabetes mellitus and macrovascular disease.