摘要
目的比较原发干燥综合征(pSS)、系统性红斑狼疮(SLE)患者外周血浆中循环DNA(cDNA)浓度的差异性,探讨cDNA浓度在pSS、SLE诊治中的意义。方法选用人类基因组管家基因甘油醛-3-磷酸脱氢酶(GAPDH)设计引物,测定pSS、SLE及健康对照组中血浆cDNA浓度,同时测定血沉(ESR)、C-反应蛋白(CRP)、免疫球蛋白(IgA、IgG、IgM)并评定不同疾病的活动指数和损害指数,分析血浆cDNA浓度与疾病活动性、相关参数的关系。结果 pSS组cDNA浓度[(368.24±196.68)ng/ml]明显高于SLE组[(244.51±164.10)ng/ml]及健康对照组[(24.56±16.76)ng/ml],差异均有统计学意义(均P<0.05);pSS组中的cDNA浓度值与干燥综合征活动指数(SSDAI)呈正相关(P<0.05),与干燥综合征损害指数(SSDDI)无相关性(P>0.05);SLE组中cDNA浓度值与SLE活动指数(SLEDAI)无相关性(P>0.05)。结论 cDNA浓度可作为pSS与SLE鉴别指标之一,pSS患者cDNA浓度与SSDAI呈正相关,可作为pSS疾病活动性观察指标之一。
Objective To investigate the plasma circulating DNA （cDNA）levels in patients with primary Sjogren＇s syn-drome（pSS） and systemic lupus erythematosus（SLE）, and its clinical significance. Methods The primer was designed by human genome housekeeper gene GAPDH. Levels of cDNA, erythrocyte sedimentation rate （ESR）, C- reaction protein （CRP） and im-munoglobulin （IgA, IgG and IgM） in each group were detected. Meanwhile, the activity index and damage index of different dis-eases were assessed, and the relations between cDNA and disease activity were analyzed. Results The levels of plasma cDNA in pSS group （368.24±196.68ng/ml） were apparently higher than those in SLE group （244.51±164.10ng/ml） and normal control group （24.56±16.76ng/ml）,and the differences were significant statistical y （both P〈0.05）.The levels of cDNA in each disease group were significantly higher than those in normal control group （both P〈0.05）.In pSS group：cDNA level was positively corre-lation with Sjogren＇s syndrome disease activity index （SSDAI）（P〈0.05）.But there was no correlation between cDNA concentration and Sjogren＇s syndrome disease damage index （SSDDI）（P〉0.05）. In SLE group：cDNA level did not correlate with SLE disease activity index（SLEDAI）（P〉0.05）. Conclusion The cDNA level may be a valuable indicator for distinguishing pSS from SLE.And it can be taken as one of the disease activity indices of pSS.
出处
《浙江医学》
CAS
2013年第18期1637-1639,1691,共4页
Zhejiang Medical Journal
基金
嘉兴市科技计划项目(2013AY21043-11)
