茶树杂交一代儿茶素类和生物碱的遗传变异分析

Genetic Variation of Catechins and Alkaloids in First Generation of Tea Plants

对茶×白毛茶人工杂交一代229个单株儿茶素类和生物碱采用高效液相色谱法进行检测。结果表明，茶树杂交一代生化成分存在丰富的多样性和变异，平均遗传多样性指数达到2.02，平均变异系数达到27.51%。F1个体的儿茶素品质指数为348.39~2583.96，平均值为762.47。通过多变量的主成分分析，前5个主成分代表了茶树生化成分多样性86.94%的信息。基于生化成分，将亲本及229个单株聚为2个类群，第1类群100个单株，第2类群131个单株，包括2个亲本。对茶树人工杂交后代儿茶素类和生物碱遗传规律的深入研究，可为茶树育种鉴定与利用提供参考。

The contents of catechins and alkaloids of 229 individuals in the combination of Camellia sinensis （L.） O. Kuntze ×C. sinensis var. pubilimba Chang were detected by using high performance liquid chromatographic （HPLC）. The results showed that the biochemical diversity and variation were high in first generation of tea plants. The average diversity index and coefficient of variation were 2.02 and 27.51%, respectively. The catechin quality index ranged from 348.39~2 583.96, with average of 762.47. In principal component analysis, the first five principal components represented 86.94%of the biochemical diversity. Based on the biochemical data, 229 individuals of first generation and parents were clustered into two groups, 100 individuals in group one, 131 individuals in group two, and both parents were clustered in group two. It provided the reference for tea breeding identification and utilization in the future.