miR-19a对于去势抵抗性前列腺癌细胞的增殖及凋亡的调控

MiR-19a regulates castration-resistance prostate carcinoma cells proliferation and apoptosis

目的:探讨miR-19a在去势抵抗性前列腺腺癌中的表达及其对去势抵抗性前列腺癌细胞株PC3及DU145的增殖及凋亡的影响。方法:用定量PCR方法检测10例去势抵抗性前列腺癌细胞组织及相关癌旁的miR-19a表达,通过MTT、克隆形成、Western blotting、流式细胞仪检测增殖及凋亡及体内成瘤的方法来研究miR-19a对于去势抵抗性前列腺癌细胞增殖及凋亡的影响。结果:miR-19a在去势抵抗性前列腺癌组织中的表达相对于癌旁组织显著提高。抑制miR-19a在激素非依赖前列腺癌中的表达,可抑制细胞的增殖及集落形成并增加其凋亡。结论:在激素非依赖性前列腺癌中,miR-19a起着癌基因作用,与前列腺癌的增殖及凋亡相关。

Objective： To explore the expression of miR-19a in castration-resistant prostate cancer （CRPC） and its role in PC3 and Du145 cells proliferation and apoptosis. Methods： miR-19a levels in 10 CRPC tissues and paracancerous tissues were measured by qRT- PCR. Effects of miR- 19a in cell proliferation and apoptosis in vitro and in vivo were evaluated by MTr assay, colony formation assay, western blotting, FACS analysis of cell cycle and apoptosis, and tumor formation assay, respectively. Results： We found that miR-19a expression was significantly increased in CRPC tissues compared to paracancerous tissues. Functional analyses showed that inhibiting the overexpression of miR-19a in androgen-independent cell lines increased their aoootosis. Conclusions： miR-19a may play as a oncogene in the development of CRPC.