阳和汤加减调节强直性脊柱炎T细胞功能及骨代谢指标的探讨

Study on the Regulation of T Cell Function of Ankylosing Spondylitis and Bone Metabolism with Yanghe Variant Decoction

目的:观察阳和汤加减的调节免疫、抑制关节炎症及骨质破坏的作用,初步从细胞、分子水平分析其部分作用机制。方法:采用蛋白聚糖+弗氏佐剂+寒湿因素病证结合的方法制造AS肾虚寒湿型动物模型。采用免疫组织化学技术SP法检测肿瘤坏死因子-α、基质金属蛋白酶-9以及RANKL、转化生长因子-β在骨组织中的表达情况。结果:①复方组、桂枝组、麻黄组、鹿角片组与模型组比较,差异有统计学意义(P<0.05),中药组均发挥疗效。②复方组、桂枝组与模型组比较,差异有统计学意义(P<0.05),复方组与桂枝组药效突出。③复方组与桂枝组、麻黄组、鹿角片组比较,差异有统计学意义(P<0.05),复方组疗效优于其他中药组。结论:阳和汤能够从多环节、多方面发挥调节免疫、抑制关节炎症及骨质破坏作用,达到良好的治疗效果。

Objective：By analysis the expression of tumor necrosis factor-α,matrix metalloproteinases-9,RANKL and transforming growth factor-β in bone tissues to observe the functions of Yanghe Variant Decoction in regulating immunity,inhibiting arthritis and the destruction of bone,making a preliminary analysis of its mechanism from the cell,molecular level.Methods：Ankylosing spondylitis animal models with asthenia renal cold were made by the combination of proteoglycan,Freund＇s adjuvant and cold damp factors.Tumor necrosis factor-α,matrix metalloproteinases-9,RANKL and the expression of transforming growth factor-β in bone tissue were detected by immunohistochemistry technique SP.Results：①Comparing the compound group,the guizhi group,the mahuang group,the lujiaopian group with the model group,the difference was statistically signiifcant（P〈0.05）,and all the Chinese medicine groups had curative effects.②Comparing the compound group,the guizhi group with the model group,the difference was statistically signiifcant（P〈0.05）,and the compound group and the guizhi group had prominent efifcacy. ③ Comparing the compound group with the guizhi group,the mahuang group and the lujiaopian group,the difference was statistically signiifcant（P〈0.05）,and efifcacy of the compound group was better than the other traditional Chinese medicine groups.Conclusion：Yanghe decoction could from many aspects regulated immunity and inhibited joint inlfammation and bone destruction,bring about good treatment results.