乙型脑炎病毒致弱株的筛选及其生长特性

ATTENUATION AND CHARACTERIZATION OF JAPANESE ENCEPHALITIS VIRUS

本文将乙型脑炎病毒(Japanese encephalitis virus,JEV)基因I型HEN0701株在BHK-21细胞上传代,并通过空斑纯化和接种小鼠来筛选弱毒株。通过空斑试验和一步生长曲线,比较弱毒株和其亲本强毒株的生长特性。结果显示,JEV HEN0701株经过在BHK-21细胞上传代100代和10轮空斑纯化,挑选获得6株克隆株。将6株克隆株经脑内和皮下接种小鼠,筛选获得一株致弱株10S3。与HEN0701相比,10S3的空斑形态比较一致;而生长曲线显示,HEN0701在BHK-21上生长速度比10S3快。

The genotype I （GI） strain HEN0701 of Japanese encephalitis virus was serially passaged up to 100 times and plaque cloned 10 times in BHK-21 cells. The attenuated strain 10S3 was identified via pathogenecity tests in mice. With a little difference in plaque size, the 10S3 strain showed more uniform plaque morphology than its parent HEN0701. The growth kinetic of the 10S3 strain was different from that of HEN0701.