油茶籽油抗氧化及对自由基清除作用研究

Research on Antioxidant and Free Fadical Scavenging Effects of Camellia Seed Oil

以贵州册享农家油茶籽油为原料,添加不同浓度的茶多酚(TP)、TBHQ以及不添加为对照,贮藏在(62±2)℃的温度条件下,观察贮藏过程中酸值、过氧化值(POV)的变化;同时提取不同处理油茶籽油中多酚类物质测定贮藏前后总酚、抗氧化能力及对自由基的清除作用。结果表明:茶多酚的抗氧化效果高于TBHQ及对照组,随着添加浓度的升高而增加。高温贮藏后从油茶籽油中提取的酚类物质具有很强的抗氧化能力;对DPPH、羟基自由基和超氧阴离子自由基有良好的清除作用。其中0.02%TP>0.03%TP>0.04%TP>TBHQ>空白。

This paper regards Guizhou Ceheng farming- Camellia seed oil as raw material, adding respectively concentration to the tea polyphenol （TP） . TBHQ as the contrast with the blank group, storing at （62_＋ 2）~C, observing the changes of acid value and peroxide value （POV）, simultaneously extracting the polyphenols from the different tea seed oils observed before and after storage of total phenolic content, the antioxidant capacity and the effect of scavenging free radicals .The results show that ： the antioxidant effect of tea polyphenol is better than that of TBHQ and the blank group. With the addition of tea polyphenol concentration, it increases. The phenolic substances which extracted from tea seed oil has strong antioxidant capacity after storaging at the high temperature , having better scavenging effects on DPPH, hydroxyl radical and superoxide anion free radicals, in which the effects were shown as 0.02 %TP 〉 0.03 %TP 〉 0.04 %TP 〉 TBHQ 〉 blank.