聚醚醚酮基纳米复合材料表面口腔微生物黏附与成膜的实验研究

Research of oral microbial adhesion and biofilm formation on the surface of Poly (ether-ether-ketone) based nanocomposites

目的制备聚醚醚酮(PEEK)基纳米复合材料,研究材料表面口腔微生物黏附与生物膜形成情况。方法分别制备PEEK基纳米羟磷灰石(n-HA/PEEK)和PEEK基纳米氟磷灰石(n-FA/PEEK)复合材料,以纯钛(CpTi)和PEEK作对照,应用微生物活性剂检测试剂盒和激光共聚焦显微镜研究PEEK基纳米复合材料表面口腔微生物黏附和成膜情况。结果与CpTi相比,黏附初期2小时内PEEK及PEEK基纳米复合材料表面口腔微生物黏附量显著减少;四组材料表面在第14天形成的生物膜形貌和厚度基本一致,但PEEK基纳米复合材料组表面生物膜内死菌/活菌比显著高于CpTi及PEEK组,而且n-FA/PEEK组显著高于n-HA/PEEK组。结论 PEEK基纳米复合材料表面细菌黏附量低于CpTi,生物膜死菌量显著高于CpTi,提示材料的成分和表面粗糙度影响口腔微生物的黏附和生物膜的组成结构,PEEK基纳米复合材料具有良好的临床应用前景。

Objective To study the oral microbial adhesion and biofihn formation on the surface of the poly （ether-ether- ketone） （PEEK） based nanocomposites. Methods With the commercial pure titanium （CpTi） and PEEK as the control groups, the bacterial adhesion and biofilm formation on the surfaces of the PEEK based nano-hydroxyaptite （n-HA/PEEK ） and PEEK based nano-fluorapatite （n-FA/PEEK）, were investigated by microbial viability assay kit and confocal laser scanning microscope. Results Compared with CpTi, the amount of early adhesion of oral microbes on the surface of PEEK and its nanocomposites at 2h was significantly decreased. On 14 days, the biofihns of all materials surface had similiar morphology and thickness, but PEEK based nanocomposites surface had significantly higher dead-to-live bacteria ratio than CpTi and PEEK, especially that n-FA/PEEK was much higher than n-HA/PEEK. Conclusion Compared with CpTi, less bacteria adhesion and more dead baceria on the surfaces of PEEK based nanocomposites suggested that the ma-terial composition and surface roughness influence the oral microbial adhesion and biofilm structure. PEEK based nan- ocomposites show a good prospect of clinical application as bone implant materials.