摘要
目的建立一种新型大鼠脊髓半切模型,为临床脊髓空洞损伤研究提供适宜的动物模型。方法 25只SD大鼠随机分为脊髓楔型块状半切组(A组,n=10)、假手术组(B组,n=10)和正常对照组(C组,n=5)。术后采用BBB运动功能评分、斜板试验、HE染色和核磁共振(MRI)检测综合评价脊髓半切模型的稳定性。结果术后A组大鼠伤侧后肢BBB运动功能评分及斜板维持最大角度值显著低于B组和C组(P<0.05),而这些指标在B组和C组之间没有明显差异;术后28 d HE和MRI检测显示脊髓损伤区(A组)灰质和白质发生明显的形态学改变。结论通过该方法建立的脊髓半切模型稳定、可靠,适于脊髓空洞性损伤的研究。
Objective Establishment a novel hemisected spinal cord injury model with rats to supply model for clinical research.Method 25 SD rats were randomly divided into wedged-block hemisected group （A group,n =10）,sham operation group （B group,n =10） and control group （C group,n =5）.After operation,the stability of the model was comprehensive evaluated by grading the scores of Basso-Beattie-Bresnahan （BBB）,swash plate test,HE staining and MRI assay.Result After operation,the mean scores of BBB in injured hind limb and maximum angle of swash plate in group A were significantly lower than group C and B （P ＜ 0.05）,but these parameters without different between B group and C group; 28 days after operation,HE staining and MRI assay shows a significant morphological changes in spinal cord tissue of group A; Conclusion The results suggested that the rat model established by this method with high quality of stability and reliability which suitable to evaluate the spinal cord injury research.
出处
《实验动物科学》
2013年第4期13-17,共5页
Laboratory Animal Science
基金
国家自然科学基金项目(No.81171467)
黑龙江省卫生科技计划项目资助(No.2010-241)
关键词
脊髓楔型半切
动物模型
大鼠
wedge-shaped spinal cord hemisection
model
rats
