HPLC法测定小儿氨酚匹林片含量的方法改进

A Better Method for Determination of Pediatric Aspirin and Paracetamol Tablets by HPLC

目的改进HPLC测定小儿氨酚匹林片含量的方法。方法色谱柱:SHIMADZU Shim-pack VP-ODS(4.6 mm×150 mm,5μm)。流动相:磷酸盐缓冲液(取0.01 mol.L-1磷酸二氢钾溶液,用磷酸调pH值至2.6±0.1)-甲醇(70∶30),检测波长:275 nm,流速:0.8 ml.min-1,进样量:20μl,柱温:30℃。结果对乙酰氨基酚、阿司匹林检测浓度分别在6.89～41.33μg.ml-1,r=0.9999;11.96～71.78μg.ml-1,r=0.9999,范围内与峰面积线性关系良好。平均加样回收率对乙酰氨基酚为99.27%,RSD为0.65%(n=6)、阿司匹林为99.59%,RSD为0.40%(n=6)。结论本方法测定操作简便,结果准确,精密度好,优于原方法,可用于小儿氨酚匹林片的含量测定。

Objective To establish an HPLC method for determination of contents in pediatric aspirin and paracetamol tablets.Methods The chromatographic column was SHIMADZU Shim-pack VP-ODS（4.6 mm×150 mm,5 μm） and the mobile phase consisted of phosphate buffer solution（the pH of 0.01 mol · L-1 potassium dihydrogen phosphate solution was adjusted to 2.6±0.1 with phosphoric acid）-methanol（70∶ 30）.The photodiode array detector was used at 275 nm and the flow rate was 0.8 ml · min-1.The injection volume was 20 μl and the column temperature was 30 ℃.Results The concentration of paracetamol and aspirin was linear in the range of 6.89-41.33 μg · ml-1（r=0.9999）,11.96-71.78 μg · ml-1（r=0.9999）.The average recovery of paracetamol was 99.27% while RSD was 0.65%（n=6）.The average recovery of aspirin was 99.59% while was 0.40%（n=6）.Conclusion This improved method is simple,accurate,and can be used for determination of content in pediatric aspirin and paracetamol tablets.