食管鳞癌组织中磷脂酰肌醇3-激酶和血管内皮生长因子的表达及其临床意义

Expression and significance of phosphoinositide 3-kinase and vascular endothelial growth factor proteins in esophageal squamous cell carcinoma

目的研究磷脂酰肌醇3-激酶(PI3K)和血管内皮生长因子(VEGF)在食管鳞癌组织(esophageal squamous cell carcinoma,ESCC)中的表达及其与食管鳞癌细胞发生、发展与转移的关系以及两者之间的相关性。方法应用免疫组织化学链菌素亲生物素-过氧化酶连接法(SP)法检测24例正常食管黏膜、94例食管鳞癌组织中PI3K和VEGF的表达情况。结果食管癌组织中PI3K和VEGF的阳性表达率与正常食管黏膜组织相比差异均有统计学意义,分别为71.3%(67/94)vs 4.2%(1/24)、61.7%(58/94)vs 0(均P<0.01);在淋巴结转移阳性组两者的表达显著高于淋巴结转移阴性组,分别为92.0%(46/50)vs 47.7%(21/44)、76.0%(38/50)vs 45.4%(20/44)(均P<0.01)。VEGF的表达与肿瘤组织类型、浸润深度等因素有关(P<0.01);PI3K与VEGF表达有关联性(C=0.311,P<0.05)。结论 PI3K、VEGF表达与食管癌发生发展及侵袭转移有关,可作为评价食管鳞癌生物学行为的指标。

Objective To study the expression of phosphoinositide 3-kinase（PI3K）and vascular endothelial growth factor（VEGF）in esophageal squamous cell carcinoma（ESCC）and to investigate the relationship among the expression of PI3K and VEGF,carcinogenesis and progression of esophageal carcinoma.Methods PI3K and VEGF expression were detected in 24cases of normal esophageal mucosa,94 cases of primary tumor tissues with streptavidin peroxidase conjugated method（SP）immunohistochemical method.Results There were significant differences of PI3K and VEGF expressions between esophageal carcinoma and normal mucosa epithelium,71.3%（67/94）vs 4.2%（1/24）,61.7%（58/94）vs 0（both P0.01）.Significant difference existed in the expression of PI3K,VEGF and the metastasis of lymph node 92.0%（46/50）vs 47.7%（21/44）,76.0%（38/50）vs 45.4%（20/44）（both P 0.01）.The positive expression rate of VEGF had the relationship with pathological differentiation invasive depth（P 0.01）.Correlation existed between PI3Kand VEGF expression（C=0.311,P0.01）.Conclusion The over expressed PI3K and VEGF play important roles in carcinogenesis and progression of esophageal carcinoma and can be used as valuable biomarkers to evaluate biological characteristics in esophageal carcinoma.