摘要
鳃隐鞭虫Cryptobia branchialis常寄生在鱼鳃上,易导致鱼类窒息死亡。中性红是一种易氧化分解的常用活体生物染色剂。本研究以普通水螅Hydra vulgaris为材料,使用中性红溶液,探索有效清除水螅体表鳃隐鞭虫的安全浓度与使用方法。结果表明：中性红浓度20~30 mg·L-1（25℃±1℃）时10 min可有效清除鳃隐鞭虫,且不影响水螅的正常生长。中性红浓度为20~50 mg·L-1时,触手出现解体的对应时间为30-10 min。研究表明,中性红溶液浓度与处理时间科学组合,能达到安全高效杀灭水螅体表寄生原虫的目的。
Cryptobia branchialis normally parasitizes in fish gills,which can cause fish death of suffocation.Neutral red is a common staining reagent of living creatures that can be easily oxidised and decomposed.In this study,the safe concentration and application method of neutral red solution which could effectively remove C.branchialis from the surface of Hydra were investigated.The results showed that C.branchialis could be removed by neutral red at the concentration of 20 ~ 30 mg·L-1（25℃ ±1℃） in 10 min without any effect on Hydra growth.When the concentration of neutral red was 20 ~ 50 mg·L-1,tentacles of Hydra could be disintegrated in 30 ~10 min.In conclusion,properly application of neutral red at designed concentration and processing time can safely and efficiently kill the parasitic protozoa on the surface of Hydra.
出处
《四川动物》
CSCD
北大核心
2013年第5期751-754,F0003,共5页
Sichuan Journal of Zoology
基金
深圳大学实验室开放基金项目
2012深圳大学教学研究B类项目("专题研究型"课题)资助
关键词
水螅
鳃隐鞭虫
中性红
安全浓度
Hydra vulgaris
Cryptobia branchialis
neutral red
safe concentration
