番茄灰霉病菌拮抗菌D6和D10发酵条件的优化及其抑菌效果

Optimized fermentation conditions of antagonistic strains D6 and D10 and their inhibitory effect to Botrytis cinerea

【目的】优化番茄灰霉病菌拮抗菌的发酵条件,初步探究其抑菌效果,为番茄灰霉病菌的生物防治提供潜在的资源菌。【方法】以前期从大蒜鳞茎中筛选出的拮抗菌D6和D10为研究试材,以接种量、温度、摇床转速和装液量为考察因素,设计四因素三水平正交试验,对D6和D10的发酵条件进行优化,并研究优化条件下D6和D10的无菌滤液对番茄灰霉病菌菌丝生长的抑制作用。【结果】D6和D10的最佳摇床培养条件分别为,D6:接种量4%,温度22℃,转速180r/min,装液量50mL;D10:接种量4%,温度26℃,转速150r/min,装液量75mL。当D6的无菌滤液质量分数达到15%时,其抑制率最高达到85.33%;当D10的无菌滤液质量分数达到10%时,其抑制率最高达到70.67%。在121℃时,D6无菌滤液的热稳定性较好,而D10的无菌滤液却失去了生物活性。【结论】在优化条件下培养的D6和D10无菌滤液对番茄灰霉病菌有一定的抑制作用,D6的无菌滤液热稳定性较好。

[Objective] This study optimized the fermentation conditions of antagonistic strains to Bot- rytis cinerea ,and explored their inhibitory effect preliminarily. [Method] D6 and D10 strains were isolated from the garlic bulb in earlier stage. Inoculum density, culture temperature, shaker revolution and loading volume as investigated factors, 4 factors and 3 levels of orthogonal experimental were designed for opti- mized fermentation conditions of D6 and D10. Their optimum shaker cultivation conditions and germfree fermented filtration inhibitory effect on B. cinerea mycelial growth were investigated. [Result] The ob- tained optimum shaker cultivation conditions were. D6 inoculum density 4%, culture temperature 22 ~C, shaker revolution 180 r/min and loading volume 50 mL and D10.inoculum density 4% ,culture temperature 26 ~C ,shaker revolution 150 r/min,and loading volume 75 mL. Highest inhibitory rates were up to 85.33% with the filtrate concentration of 15% and 70.67% with the filtrate concentration of 10%. When the tem- perature was 121 ℃ ,the filtration of D6 was stable,while D10 lost biological activity. [Conclusion] Germ-free fermented filtrations of D6 and D10 had a striking inhibitory effect to B. cinerea. D6 showed good ther- mal stability and should be further studied.