内蒙古地区疑似强直性脊柱炎患者HLA-B27抗原阳性率观察

Investigation of positive rate of HLA-B27 antigen in patients with suspected ankylosing spondylitis in Inner Mongolia

目的通过检测998例疑似强直性脊柱炎（Ankylosing spondylitis,AS）患者HLA-B27抗原阳性率和表达强度,了解内蒙古地区AS与HLA-B27抗原的相关性,并初步探讨其临床意义。方法采用流式细胞术检测患者外周血T淋巴细胞膜上的HLA-B27抗原和其平均荧光强度（mean fuorescence intensity,MFI）。结果 998例疑似AS患者以21~50岁青壮年为主,占77.35%;男女比例为1.4∶1;998例疑似AS患者的HLA-B27抗原阳性率28.86%;男女患者HLA-B27抗原阳性率分别为33.05%和23.02%（P〈0.05）;男女阳性患者的MFI有明显差异（P〈0.05）。结论内蒙古地区AS患者与HLA-B27抗原有着很强的相关性。采用流式细胞技术检测临床疑似AS患者T淋巴细胞HLA-B27表达强度有助于诊断和鉴别诊断AS。

Objective To investigate the relationship between suspected ankylosing spondylitis and HLA-B27 antigen in Inner Mongolia by detecting the positive rate and expression intensity of HLA-B27 antigen in 998 suspected AS patients,and evaluate its clinical signifcance.Methods The positive rate and the mean fuorescence intensity（MFI） of HLA-B27 antigen on the peripheral blood T lymphocytic detected by fow cytometer.Results Among the 998 suspected AS patients,the majority patients（77.35%） were aged from 21 to 50 years old,and the ratio between male and female was 1.4 ∶ 1.Among the all patients,the positive rate of antigen HLA-B27 was 28.86%,and the male and female patients＇ positive rates of HLA-B27 antigen were 33.05% and 23.02%,respectively（P0.05）.The MFI of male and female patients were signifcantly different（P0.05）.Conclusion The patients with AS in Inner Mongolia are strongly associated with HLA-B27 antigen.Detection of HLA-B27 antigen expression intensity in suspected AS patients with FCM is helpful to diagnosis and differential diagnosis of AS.