产类黄酮链霉菌的紫外线诱变选育

Breeding of yielding flavonoids Streptomyces by UV Mutation

诱变选育高产类黄酮链霉菌菌株,采用不同强度的紫外线对出发菌株链霉菌HNS2-2单孢子悬液诱变处理。以出发菌株作为对照,分光光度法测定诱变菌株发酵产物黄酮类物质含量为评价指标,进行反复筛选。在20 W紫外灯、辐射距离30 cm、照射20 s条件下,获得高产菌株251,其黄酮类物质产量达41.87μg/mL,比出发菌株提高15.52%,且连续7次传代稳定性能稳定。通过紫外诱变选育的方法,能提高出发菌株黄酮类物质产量。

A stable,high flavonoids-producing Streptomyces strain is screened.Streptomyces HNS2-2 as the original strain is treated by ultraviolet（UV） mutagenesis,high-yield mutant is obtained by fermentation,determination of flavonoids and screening.Under 20 W UV-lamp,distance 30 cm,irradiation 20 s,the production output of high-yield mutant 251 reached 41.87 μg/mL,15.52% higher than the original strain.And determination of genetic stability showes that the hereditary character of it is stable.This method is ideal to improve the flavonoids yield of original strain.