摘要
诱变选育高产类黄酮链霉菌菌株,采用不同强度的紫外线对出发菌株链霉菌HNS2-2单孢子悬液诱变处理。以出发菌株作为对照,分光光度法测定诱变菌株发酵产物黄酮类物质含量为评价指标,进行反复筛选。在20 W紫外灯、辐射距离30 cm、照射20 s条件下,获得高产菌株251,其黄酮类物质产量达41.87μg/mL,比出发菌株提高15.52%,且连续7次传代稳定性能稳定。通过紫外诱变选育的方法,能提高出发菌株黄酮类物质产量。
A stable,high flavonoids-producing Streptomyces strain is screened.Streptomyces HNS2-2 as the original strain is treated by ultraviolet(UV) mutagenesis,high-yield mutant is obtained by fermentation,determination of flavonoids and screening.Under 20 W UV-lamp,distance 30 cm,irradiation 20 s,the production output of high-yield mutant 251 reached 41.87 μg/mL,15.52% higher than the original strain.And determination of genetic stability showes that the hereditary character of it is stable.This method is ideal to improve the flavonoids yield of original strain.
出处
《农产品加工(下)》
2013年第9期1-3,共3页
Farm Products Processing