HPLC法测定心可舒片中的丹参酮成分含量

Determination of Tanshinones in Xinkeshu tablets by HPLC

目的测定心可舒片中二氢丹参酮I、隐丹参酮、丹参酮I和丹参酮Ⅱ^含量。方法采用Kromasil．C（5μm，4．6mmx250ram）色谱柱；乙腈-0．1％甲酸（60：40，v／v）为流动相，流速为1．0mL·rain～；紫外检测波长为270nm；柱温30℃。结果二氢丹参酮I在线性范围0．0523—5．23μg·mLll（r=0．9997）、隐丹参酮在线性范围0．0312—3．12μg·mLll（r=0．9999）、丹参酮I在线性范围0．0204～2．04μg·mLll（r=0．9998）及丹参酮Ⅱ^在线性范围0．0617～6．17μg·mL-1（r=0．9999）都具有良好的线性关系，平均回收率分别为98．72％、99．49％、99．01％和98．98％，4种化合物的日内精密度RSD分别为0．84％、0．59％、0．95％和0．78％。结论该方法快速、准确、重复性好，可同时定量心可舒片中4种脂溶性成分。

OBJECTIVE To estabilish an HPLC method for quantitative determining of dihydrotanshinone I , cryptotanshinone,tanshinone I and tanshinone 11 A in Xinkeshu tablets. METHODS Separation was accomplished on the Kromasil-C~ column （5txm,4. 6mm x 250mm）, with acetonitrile-0. 1% formic acid（60： 40, v/v） as mobile phase. The flow rate was 1.0mL ~ min-1 and the detection wavelength was set at 270nm. The column temperature was at 30~C. RESULTS The method offered good linearity 0. 0523 ~ 5. 231xg ~ mL-1 for dihydrotanshinone I （ r = 0. 9997 ） ,0. 0312 ~ 3. 121xg ~ mL-1 for eryptotanshinone （ r = 0. 9999 ） ,0. 0204 ~ 2. 04txg ＂ mL-1 for tanshinone I （r=0.9998） and 0.0617 ~ 6. 171xg ~ mL-1 for tanshinone 11A （r = 0.9999）. The average recovery rate was 98.72% ,99. 49% ,99.01% and 98.98% and the intra-day precision RSDs was 0. 84% ,0. 59% ,0. 95% and 0. 78% respectively. CONCLUSION The method is rapid, accurate credible and repeatable, and can be used for quality control at the same time in the fat-soluble components.