树脂法提取海洋微生物小分子代谢产物的研究

Studies on extraction of small molecule metabolites produced by marine microorganisms with macroporous adsorption resin

目的采用大孔吸附树脂建立海洋微生物小分子代谢产物库的粗提取方法。方法以吸附率、解吸率、蛋白与总糖吸附率、指纹图谱为评价目标,对常用的11种不同极性的大孔吸附树脂进行筛选。结果 DM11与SD300串联吸附对发酵液中化合物的吸附率达7.528%,总糖与蛋白去除率分别为90.98%和77.69%,且此法几乎不损失乙酸乙酯萃取部分化合物。结论 DM11-SD300串联树脂吸附法能快速有效地提取发酵液中的小分子化合物,为海洋微生物小分子代谢产物库的建立提供了实验基础。

OBJECTIVE To establish a crude extraction method for the library of small molecule metabolites produced by marine microorganisms using maeroporous adsorption rosin （MAR）. 1VIETI-IEDS 11 types of MARs were selected based on their adsorption rate, desorpfion rate, protein and polysaecharide adsorption rate. RF^ULTS DMll and SD300 in series, were used to extrat small chemical molecules from bacterial fermentation liquid, by which the adsorption rate is up to 7.528% ,while the removal rate of protein and polysaeeharides is 90.98% and 77.69% respectively. In addition, this process barely lose compounds that extracted from ethyl ace= rate. CONCLUSION the approach of DM11-SD300 in series could extract small molecule metabolites from fermen= tation liquid rapidly and effectively,which will make a fundation to achieve the library of small molecule metabolites produced by marine microorganisms.