摘要
目的观察福建省慢性丙型肝炎患者基因型分布特点以及与患者性别、年龄、丙型肝炎病毒(HCV)RNA之间的关系。方法应用反转录聚合酶链反应(RT-PCR)和SANGER测序法对155例慢性丙型肝炎患者的HCV进行基因分型。结果在155例标本中,HCV基因1型占55.48%,基因2型占18.71%,基因3型13.51%,基因6型12.26%,未见基因4、5型;男女慢性HCV感染均以1型为主要基因型,在其他基因型中,男性3型、6型多于2型,女性则以2型为主,差异有统计学意义(P<0.05);基因2型平均年龄为(49岁),3型平均年龄为(35岁),差异有统计学意义(P<0.05)。结论福建地区HCV基因型以1b型为主,其次是2a型,未见基因4、5型;基因2型的感染者平均年龄较基因3型大。
Objective To study the distribution of hepatitis C virus（HCV）genotypes in patients with chronic hepatitis C（CHC）in Fujian and the relationship with gender,age and serum HCV RNA level.Methods HCV genotypes were detected 155 CHC patients by using reverse transcript polymerase chain reaction（RT-PCR）and SANGER sequencing.Results The percentages of HCV genotype 1,2,3and 6were 55.48%,18.71%,13.51%and 12.26%.Genotype 1was the most common genotype in both male and female CHC patients.Percentages of genotype 3and 6were higher than genotype 2in male patients,and genotype 2was more common in female patients（P 0.05）.The average age of patients with genotype 2or 3were 49and 35years old,with statistical difference between them（P0.05）.Conclusion HCV genotype 1bcould be the most common genotype in CHC patients of Fujian,followed by genotype 2a,and the average age of patients with genotype 2infection might be younger than patients with genotype 3infection.
出处
《检验医学与临床》
CAS
2013年第19期2506-2507,共2页
Laboratory Medicine and Clinic
基金
福州市科技发展基金资助项目(2008-S-73)
