摘要
探讨了粘红酵母(Rhodotorula glutinis31229)和红酵母菌株RY-01混合发酵产β-胡萝卜素的影响因素。实验结果表明,两株红酵母混合发酵的β-胡萝卜素产量比单菌发酵显著提高,达到8837.83μg/L,菌体生物量也达到了23.56g/L;β-胡萝卜素产量的积累与菌体生物量的积累并不同步,在时间上有一定的滞后性;混合发酵的最适碳源和氮源分别是麦芽糖和蛋白胨;K+和Mg2+对β-胡萝卜素的产量有一定的影响作用,但不显著;橘皮干粉浸汁对混合发酵菌体生物量和β-胡萝卜素产量有较大的促进作用,分别提高了41.3%和52.8%。本研究为β-胡萝卜素高产菌株的选育和β-胡萝卜素的进一步开发利用提供了参考。
The influence factors of β-carotene production by co- fermentation of Rhodotorula glutinis and Rhodotorula RY-Oi were studied. The results showed that the production was significantly improved by co- fermentation of two strains than single strain fermentation. β-carotene production reached 8837.83μg/L and the yeast biomass was 23.56g/L.The accumulation of β-carotene was not synchronized with biomass accumulation, which was lag behind the biomass.The optimum carbon source and nitrogen source of co-fermentation medium was maltose and peptone respectively.The β-carotene production was not significantly improved by K + and Mg2+. The extract of orange peel powder enhanced yeast biomass and β-carotene production,which were promoted by 4].3% and 52.8% respectively.This paper would offer references to screen the rhodotorula producing β-carotene and viable utilization of (3-carotene.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第21期157-161,共5页
Science and Technology of Food Industry
基金
2012年度安徽师范大学创新基金项目(2012cxjj04)
安徽师范大学教学研究项目(201013)
大学生创新性实验计划项目(201210370089)
培育计划项目(pyjh2012196)
2012年国家级大学生创新性实验计划项目
省级重点实验室与省级重点学科开放基金
优秀创新团队建设计划的基金资助
教育部高等学校博士学科点专项科研基金(20123424110004)资助