正交实验设计优化芦笋嫩茎多酚的提取工艺

Optimization of the extraction of polyphenolics from young stems of Asparagus officinalis using orthogonal array design

以芦笋嫩茎为研究材料,采用单因素和正交实验设计对芦笋多酚提取工艺进行优化,考察乙醇浓度、提取时间、提取温度以及料液比对芦笋多酚提取率的影响。结果显示,芦笋多酚提取的最佳工艺条件为,料液比1∶15、50%的乙醇、80℃水浴条件下提取60min,在该条件下,芦笋多酚的得率为6.90%。该最佳工艺费用低,操作简便,可为芦笋的加工利用提供技术上的支持。

While the young stems of Asparagus officinalis were used as the research object,the optimum conditions for extraction of polyphenolic were investigated by the single factors and orthogonal array design experiment,and the effect of ethanol concentration,extraction time,extraction temperature and solid-to-solvent ratio on polyphenol yield were studied.The result showed that the optimal conditions were as follows：ethanol concentration 50%, solid- to-solvent ratio 1：15, extraction temperature 80℃ and extraction time 60min. Under this condition, the extraction yield could reach 6.90%.The method was low-cost and easy to operate,thereby could provide technical support for the processing and utilization of asparagus officinalis.