普洱茶对酒精性脂肪肝大鼠抗氧化应激作用的研究

Effect of Fermented Pu-erh Tea on the resistance to oxidative stress in rats with alcoholic fatty liver

为探讨普洱茶(熟茶)对酒精性脂肪肝大鼠抗氧化应激作用的影响,将Wistar大鼠随机分为阴性、阳性、药物对照组、普洱茶低、中、高剂量组,60d后牺牲试验大鼠,测定大鼠肝组织细胞色素P4502E1(CYP2E1)、胰岛素样生长因子1(IGF-1)基因表达情况、大鼠肝组织中氧自由基(ROS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量、大鼠血清胰岛素(INS)、游离脂肪酸(FFA)、甘油三酯(TG)水平。结果表明:与阳性对照组比较,高剂量组大鼠肝组织CYP2E1基因表达显著降低(p<0.05);高剂量组大鼠肝组织IGF-1基因表达、GSH-PX活性及中、高剂量组SOD活性显著升高(p<0.05);高剂量组大鼠肝组织中MDA含量及中、高剂量组大鼠ROS活性,低、中、高剂量组大鼠血清INS活力、TG含量,中、高剂量组大鼠血清FFA含量均显著降低(p<0.05,p<0.01)。说明普洱茶对酒精性脂肪肝大鼠具有抗氧化应激的作用。

To explore the effect of fermented Pu-erh tea on the resistance to oxidative stress in rats with alcoholic fatty liver,Wistar rats were divided into negative control group, postive control group, drug group, low, medium and high dose of the fermented Pu-erh tea group. All rats were sacrificed after 60 days＇ feeding and the genge expression of CYP2E], IGF-], liver tissue levels of ROS, SOD, GSH- PX and MDA ~ serum levels of INS, FFA and TG were observed.The experiment results showed that compared to the postive control group,the gene expression of CYP2Et in high does group of fermented Pu-erh tea was significantly decreased（p 〈 0.05） ;the gene expression of IGF-I ,GSH-PX in high does group,SOD in medium and high does group of the fermented Pu-erh tea were significantly increased（p 〈0.05）;the liver tissue levels of MDA in high group and ROS in medium and high does group,the serum levels of INS,TG in low,medium and high dose group,FFA in medium and high dose group of fermented Pu-erh tea were significantly decreased （ p 〈 0.05, p 〈 0.0] ） .Based on the above, one can draw a conclusion that fermented pu-erh tea can resistance to oxidative stress in rats with alcoholic fatty liver.