摘要
电子克隆是随着基因组计划的实施而发展起来的利用生物信息学手段进行基因克隆的新方法,已经公布的极大节旋藻GSS序列使得电子克隆极大节旋藻的功能基因成为可能。为建立极大节旋藻功能基因的高效克隆策略,应建立利用GSS序列克隆极大节旋藻功能基因的基本策略并加以应用。具体操作方法:对dbGSS数据库中的605条极大节旋藻GSS序列进行拼接及功能注释,并在分析编码区完整性的基础上设计引物并进行PCR扩增以获得基因全长序列,利用以上策略成功克隆了极大节旋藻功能基因ureaseβ和dUTPase的全长序列。
In silico clone was a new strategy of gene clone via bioinformatics, which developed with genome enforcement. Since lots of GSS sequences were published in GenBank （dbGSS） , it was possible to identify the functional genes from Arthrospira maxima via in silico clone. Briefly, the GSS sequences of A. maxima from dbGSS were clustered and analyzed via bioinformatics tool, and primers were designed based on the yield results and used in PCR reaction. Two genes, urease beta subunit and dUTPase, were cloned based on this strategy.
出处
《北京联合大学学报》
CAS
2013年第4期62-65,共4页
Journal of Beijing Union University
关键词
极大节旋藻
GSS序列
电子克隆
功能基因
Arthrospira maxima
GSS sequences
in silico clone
Functional genes
