摘要
为获得健壮的榉树组培继代芽,采用以芽繁芽的方式对榉树离体芽进行扩繁,研究不同因素对榉树无菌芽继代增殖效果的影响。结果表明:BA对芽的增殖倍数有显著影响,木质化程度较高的腋芽丛生芽采用添加BA 4.0 mg/L、KT 2.0 mg/L、NAA 0.5 mg/L的WPM培养基可获得较高的增殖率。为避免芽体愈伤化,木质化程度较低的其它3种来源的无菌芽在继代培养的前期,应在不添加BA的培养基上培养。附加2.0 g/L PVP的培养基能有效抑制组培继代芽的褐化。
To obtain robust subcultured shoots in Zelkova schneideriana, shoots in vitro were propagated by bud proliferation, and the factors influencing shoots in vitro multiplication were studied. The results show that BA has a significant effect on proliferation multiples of Z. schneideriana shoots. The highly lignified shoot can reach a high proliferation coefficient in WPM supplemented with BA 4.0 mg/L, KT 2.0 mg/L and NAA 0.5 mg/L. To avoid bud callus formation, the low lignified shoots from other three sources should be cultured in medium without BA during the earlier stage. The medium with 2,0 g/L PVP can effectively inhibit browning of subculture bud.
出处
《经济林研究》
北大核心
2013年第3期54-58,共5页
Non-wood Forest Research
基金
国家林业公益性行业科研专项“榉树优良种源选择及快速繁育技术研究”(200904011)
广西优良用材林资源培育重点实验室自主课题“榉木种源遗传多样性及器官发生技术研究”(12B0301)
广西青年基金项目“榉树小尺度遗传结构:基于广西雅长自然保护区群体的SSR研究”(2013GXNSFBA019093)
关键词
榉树
继代培养
增殖
褐化
愈伤化
Zelkova schneideriana
subculture
multiplication
browning
callus formation
