人脑胶质瘤PET成像中^(11)C-蛋氨酸摄取与细胞增殖和血管形成的关系

Relation of ^(11)C-MET uptake in PET with cell proliferation and angiogenesis in human brain gliomas

目的探讨人脑胶质瘤中11 C-蛋氨酸(11 C-MET)摄取量与细胞增殖和血管形成的关系。方法对30例新诊断的脑胶质瘤患者行11 C-MET PET检查,计算11 C-MET最大标准化摄取值(SUVmax);采用免疫组化方法检测Ki-67和CD34抗原在相同脑胶质瘤标本中的表达,计算Ki-67标记指数(Ki-67LI)和微血管密度(MVD)。结果 11 C-MET SUVmax和Ki-67LI均随胶质瘤病理级别的升高而明显增加(P=0.000,P=0.000);在高恶性度胶质瘤中11 C-MET SUVmax和Ki-67LI均明显高于低恶性度胶质瘤(P=0.000,P=0.000);而MVD在胶质瘤不同病理级别间(P=0.831)以及高、低恶性度胶质瘤间(P=0.370)均无明显差异。11 C-MET SUVmax与Ki-67LI存在明显正相关(P=0.000),而11 C-MET SUVmax与MVD(P=0.154)以及Ki-67LI与MVD(P=0.842)之间均无明显相关性。结论 11 C-MET摄取量和细胞增殖活性可较好地反映胶质瘤病理级别和恶性程度。

Objective To investigate the relation of 11C-methionine （11C-MET） uptake with cell proliferation and angiogenesis in human brain gliomas .Methods For 30 cases of newly diagnosed glioma patients ,positron emission tomography （PET ） examina-tion with 11C-MET was performed ,and the maximal standardized uptake value （SUVmax） of 11C-MET was measured .Expression of Ki-67 and CD34 antigens was examined by immunohistochemistry method in the same glioma samples ,both Ki-67 labeling index （Ki-67 LI） and microvessel density （MVD） were measured .Results Both 11C-MET SUVmax and Ki-67 LI increased significantly with glioma pathological grade ascending （P=0 .000 ,P=0 .000） ,and which in malignant glioma tissues were significantly higher than those in benign glioma tissues as well （P=0 .000 ,P=0 .000）;however ,there were not significant differences in MVD among different grades of gliomas （P=0 .831） as well as between high and low malignant gliomas （P=0 .370） .11 C-MET SUVmax was significantly positively correlated with Ki-67 LI （P= 0 .000） ,however ,there were not significant correlations between 11C-MET SUVmax and MVD （P=0 .154） as well as between Ki-67 LI and MVD （P=0 .842） .Conclusion 11C-MET uptake and cell prolif-eration activity can better reflect the pathological grades and malignant degrees of gliomas .