COPD大鼠不同分期炎性因子的差异与肾脏的相关性研究

A Probe into Differences of Inflammatory Factors and Correlation with the Kidney at Different Stages of COPD in Rats

目的:观察慢性阻塞性肺疾病(COPD)大鼠不同分期肺组织匀浆白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)含量,尿Cys-C mRNA表达水平的变化,以及肺肾病理结构的变化,探讨COPD炎症损伤对肾脏功能的影响。方法:将大鼠随机分为空白组(N)、COPD组(B)、COPD急性加重期组(C),每组8只,用改良烟熏加气管滴脂多糖(LPS)法建立COPD模型,模型完成后再次气管内滴加LPS造成COPD急性加重模型。ELISA法检测肺组织匀浆IL-8、TNF-α含量,RT-PCR法检测大鼠尿Cys-C mRNA表达。结果:B、C组与空白组相比较,肺组织匀浆中IL-8含量均显著增高(P<0.01),C组比B组含量增高(P<0.05);B、C组与空白组相比较,B组TNF-α含量比空白组增高(P<0.05),C组比空白组含量显著增高(P<0.01),C组比B组含量增高(P<0.05);B、C组与空白组相比较,尿中Cys-C mRNA表达均显著升高(P<0.01),B、C组比较表达升高(P<0.05)。结论:在COPD疾病中,肺脏炎症感染程度对肾脏功能有一定的影响,COPD可引起一定的肾损害,提示在COPD病程中肺肾之间的相关性,及COPD治疗过程中应注意对肾脏的保护。

Objective： To observe the change of content of the interleukin-8 （IL-8）, the tumor necrosis factor-α （TNF--α, the expression levels of the urinary Cys-C mRNA and the pathological changes of lung and kidney, in lung tissue homogenate of rats at different periods of chronic obstructive pulmonary disease （COPD）, to explore the impact of the COPD inflammatory damage on the kidneys function. Methods： the rats were divided into blank group （N）, COPD group （B） and acute exacerbation of COPD group （C）, 8 rats in each group. The model of COPD was established with the improved smoke plus the tracheal drops of the lipopolysaccharide （LPS）. Afterwards, drop the LPS into the endotrachea to establish the acute exacerbation model of the COPD. The content of the IL-8 and TNF-alpha in the lung homogenate were detected with the ELISA method, and the expressions of the rat urine Cys-C mRNA was detected with the RT-PCR method. Results： Compared with the blank group, the level of IL-8 in the lung homogenate all rose in COPD group （B） and acute exacerbation of COPD group （C）, and the level of IL-8 in AECOPD group （C） was higher than COPD group （B） （P〈0.05）; Meanwhile, the level of TNF--α in the COPD group （B） increased （P〈0.05）, but AECOPD group （C） increased significantly （P〈0.O1）, and AECOPD group （C） was higher than COPD group （B）（P〈0.05）; the urine Cys-C mRNA expression were significantly increased in COPD group （B） and AECOPD group （C）（P〈0.01）, and the expression level of AECOPD group （C） was higher compared with COPD group （B）. Con- clusion： In the COPD disease, the lung infection and damage of functions have certain effects on the kidney function, and COPD can cause certain damage to the kidney, all of which point out the relationship between the kidney and lung in the course of COPD.