HPLC法测定桐叶中齐墩果酸和熊果酸的含量

Determination of Oleanolic Acid and Ursolic Acid in Paulownia Leaves by HPLC

目的:建立桐叶中齐墩果酸和熊果酸的含量测定方法。方法:采用HPLC法,色谱柱为Promosil C18柱(4.6 mm×250 mm,5μm),流动相为乙睛-甲醇-水-磷酸(66∶12∶22∶0.02),流速为1.0 mL·min-1,检测波长为210 nm,柱温为25℃,进样量为20μL。结果:齐墩果酸在0.1292-1.5504μg范围内呈良好的线形关系(r=0.9999),平均回收率为100.1%,RSD%为3.4%(n=6)。熊果酸在0.2428-2.9136μg范围内呈良好的线形关系(r=0.9999),平均回收率为99.3%,RSD%为2.2%(n=6)。结论:该方法操作简单、准确,重现性好,可用于桐叶的质量控制。

Objective ：To establish a method for the determination of oleanolic acid and ursolic acid in Paulownia Leaves. Methods：HPLC was applied, and column was Promosil C18（4.6 mm×250mm, 5μm）; mobile phase was acetonitrile- methanol - water-phosphoric acid （66：12：22：0.02）;flow rate was 1.0 mL＇min-1; the detection wavelength was 210 nm; column temperature was 25 ℃; the injection volume was 20 μL. Results：Oleanolic acid in the range of 0.1296-1.5504μg showed good linear relationship （r=0.9999）,and the average recovery was 100.1%; RSD% was 3.4% （n=6）. Ursolic acid in the range of 0.2428- 2.9136 μg showed good linear relationship （r=0.9999）, and the average recovery was 99.3%; RSD% was 2.2% （n=6）. Conclusion： The method is simple, accurate, reproducible,and can be used for Paulownia Leaves quality control.