产复合酶黑曲霉变异株598的选育研究

Screening of Aspergillus niger Mutant Strain 598 with High Yield of Multienzyme

出发菌株黑曲霉 10 3,经紫外诱变获得一株复合酶高产菌株 598。简单固体发酵培养 ,其纤维素酶活达 2 80 4 2U/g(干曲 )、蛋白酶活为 15510U/g(干曲 )、果胶酶活 150 4U/g(干曲)、糖化酶活 2 4 77U/g(干曲 ) ,分别比出发菌株 10 3提高 113 9%、2 9 2 %、112 5%、115 4 %。经斜面传代培养 4代 ,产酶性能稳定。本文还对复合酶菌株的初筛方法进行了初步探讨。

A mutant 598 was obtained from wild strain of Aspergillus niger  103 by UV treatment Incubated in a solid state fermentation medium at 30℃ for 72 hours,the enzyme activities were:cellulase 28?042?U/g,acid proteinase 15?510?U/g,pectinase 1?504?U/g,glucoamylase 2?477?U/g, increasing respectively by 113 9%,29 2%,112 5% and 115 4% And the screening of mutant with yield of multienzyme was studied in this thesis