摘要
目的:探讨肌肽对高糖诱导的H9c2心肌细胞损伤的拮抗作用。方法:体外培养大鼠心肌细胞H9c2,细胞分为正常对照组、高糖损伤组和肌肽预保护组。MTT法检测细胞存活率,DCFH-DA荧光探针检测细胞内活性氧(ROS)水平,并利用Western blotting检测半胱氨酸天冬氨酸蛋白酶(caspase-8、caspase-9和caspase-3)活性片段的表达。结果:细胞存活率随着高糖刺激时间和高糖浓度的增加而逐渐降低,而肌肽预保护组细胞的存活率显著高于高糖处理组(P<0.05)。高糖组ROS水平比正常对照组明显增加(P<0.05),而肌肽预保护组与高糖组相比ROS水平降低(P<0.05)。高糖组caspase-8、caspase-9和caspase-3活性片段表达量比正常对照组显著升高(P<0.05),而肌肽预保护组与高糖组相比,caspase-9和caspase-3活性片段表达量显著降低(P<0.05),而活化caspase-8相对含量无明显变化(P>0.05)。结论:肌肽能够拮抗高糖诱导的H9c2心肌细胞氧化应激和凋亡损伤。
AIM: To explore the protective effect of carnosine (Car) on cardiomyocytes with high glucose (HG)-induced injury. METHODS:Rat H9c2 cardiomyocytes were cultured in vitro and divided into three groups: normal control (NC) group, HG group and Car pretreatment (Car+HG) group. The survival rate of H9c2 cells was measured by MTT assay. Intracellular level of reactive oxygen species (ROS) was detected by fluorescent probe DCFH-DA. The protein expression of caspase-8, caspase-9 and caspase-3 was determined by Western blotting. RESULTS:The survival rate of H9c2 cells decreased with the increases in glucose concentration and time, while pretreatment with 20 mmol/L Car could increase the survival rate significantly (P〈0.05). The intracellular level of ROS in HG group was significantly increased compared with NC group (P〈0.05), while that in Car+HG group was significantly decreased compared with HG group (P〈0.05). The expression of caspase-8, caspase-9 and caspase-3 proteins in HG group was significantly increased compared with NC group (P〈0.05). Compared with HG group, the expression of caspase-9 and caspase-3 was significantly decreased in Car+HG group (P〈0.05), but the expression of caspase-8 did not obviously change (P〉0.05). CONCLUSION:Carnosine can protect H9c2 cells against the injury of oxidative stress and apoptosis induced by high glucose.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第10期1783-1787,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金专项基金资助项目(No.31240006)
黑龙江省教育厅科研基金资助项目(No.12521630)